In the 60s and 70s of the 20th century, China selected parts of the central and western regions to carry out Third-line construction for nearly 20 years, which rapidly improved the public infrastructure services and living standards of the villages where they were located. However, with the arrival of the end of construction, the geographical environment of the Third-line factory can not meet the development needs, and the enterprises are gradually moved out, resulting in different degrees of abandonment of factory buildings, ecological pollution, and separation of urban areas and rural development, becoming the core factors restricting the development of village industries and rural revitalization. Based on the systematic deconstruction of Third-line industrial wasteland, this study deeply analyzes the logical mechanism between the redevelopment of Third-line industrial wasteland and village industrial revitalization, that is, the specific industrial revitalization goal of ecological industrialization, improvement of industrial chain, and revitalization of industrial vitality is achieved through the specific measures of village ecological restoration, land-use function re-endowment, and humanistic spirit mining. On this basis, it takes Xiayishui Village, Hanzhong City, Shaanxi Province as the research object, analyzes and summarizes the existing problems in its industrial development, and proposes that the essence of the redevelopment of Third-line industrial wasteland is to realize capital reconstruction and asset reconstruction through the restructuring of residual material resources and the revitalization of humanistic spirit, so as to realize industrial transformation optimization, industrial emerging formats and industrial structure upgrading, and summarize that through handling legacy disputes, enriching industrial types, improving spatial layout, implementing pollution control, and inheriting spiritual connotation and other specific measures finally achieve the development path of clear land rights and responsibilities, integrated development of three industries, homogenization of infrastructure, industrialization of ecological resources, and injection of new industrial vitality, and provide a certain reference for the development of village industries facing the same dilemma.