To construct the Beagle canine periodontal defect model and to observe the effect of periodontal tissue defect repairment of the periodontal membrane stem cells-dental follicle stem cells composite membrane.

Dog follicle stem cells (dDFSCs) and dog periodontal ligament stem cells (dPDLSCs) were isolated enzymatically and expanded in vitro. The dDFSCs, dPDLSCs and the mixed cells were used to make cell sheets separately. Then, three types of cell sheets were transplanted to dental root surfaces having three-wall periodontal defects in an allogeneic manner, and bone defects were filled with hydroxyaptite-tricalcium phosphatel (HA-TCP). The group only filled with HA-TCP was set as the control group. The periodontal clinical indices were evaluated at the time of 1 week and 2, 4, 6, 8 and 12 weeks after surgery. 12 weeks after surgery, the animals were sacrificed and decalcified specimens were prepared. Healing of the periodontal defects was evaluated histologically and histometrically.

Periodontal clinical examination, histological and histometrical analysis indicate that the periodontal healing of cell sheets groups were superior to the control group, and the mixed cells sheets group was superior to the other two groups (P ＜ 0. 05).

Allogeneic composite cell sheets of dDFSCs and dPDLSCs shows better effects in the repairment of Beagle periodontal tissue defect.