To discuss the feasibility of orthodontic treatment for regenerated periodontal tissues by comparing the difference of tooth movement under orthodontic forces between the normal periodontal tissues and the reconstructed periodontal tissues by the composite cell sheets of dental follicle cells (DFCs) and periodontal ligament stem cells (PDLSCs).

Six adult male beagles were selected for experiment animals. The first premolar in all four quadrants of each beagle were extracted and a bone defect were made in the same size (4 mm × 4 mm × 4 mm) distal to the extraction sockets. The upper left and the lower right quadrants were divided into the regeneration group by transplantation of the composite cell sheets while the upper right and lower left quadrants were control group without transplantation. 12 weeks after transplantation, the orthodontic forces were introduced to move the second premolar mesially with a force value 150 g. Measurements were done after 4 weeks of traction to record the movement of the tooth. And compare the difference between the regeneration group and control group by the statistical analysis.

DFCs/PDLSCs composite cell sheets repaired the periodontaltissue and formed the typical alveolar bone-periodontal membrane-cementum structurewhich shows good histological characteristics. experimental group and control group show no statistical difference in the rate of tooth movement (P＞0.05).

The periodontal tissues regenerated by this kind of the composite cell sheet showed a good response to mechanical stimulation.

To construct the Beagle canine periodontal defect model and to observe the effect of periodontal tissue defect repairment of the periodontal membrane stem cells-dental follicle stem cells composite membrane.

Dog follicle stem cells (dDFSCs) and dog periodontal ligament stem cells (dPDLSCs) were isolated enzymatically and expanded in vitro. The dDFSCs, dPDLSCs and the mixed cells were used to make cell sheets separately. Then, three types of cell sheets were transplanted to dental root surfaces having three-wall periodontal defects in an allogeneic manner, and bone defects were filled with hydroxyaptite-tricalcium phosphatel (HA-TCP). The group only filled with HA-TCP was set as the control group. The periodontal clinical indices were evaluated at the time of 1 week and 2, 4, 6, 8 and 12 weeks after surgery. 12 weeks after surgery, the animals were sacrificed and decalcified specimens were prepared. Healing of the periodontal defects was evaluated histologically and histometrically.

Periodontal clinical examination, histological and histometrical analysis indicate that the periodontal healing of cell sheets groups were superior to the control group, and the mixed cells sheets group was superior to the other two groups (P ＜ 0. 05).

Allogeneic composite cell sheets of dDFSCs and dPDLSCs shows better effects in the repairment of Beagle periodontal tissue defect.