To evaluate the inflammatory cytokine expressions of inflamed human gingival fibroblast cells (iHGFs) stimulated in 4 types of dental metal extractions.

Inflamed gingival tissues from 10 patients with periodontitis were collected, and primary iHGFs were cultured. The cells were stimulated by Co-Cr, Ti alloy, pure Ti and AuPd alloy extractions respectively, and DMEM was used as blank control. Protein productions of pro-inflammatory mediators and related mRNA expressions were measured with ELISA and RT - PCR. Statistics were tested with Wilcoxon matched-pairs test.

ELISA and RT-PCR results showed the protein and mRNA expressions of interleukin-6, interleukin-1β, tumor necrosis factor-α in Ni-Cr and Ti alloy groups were both higher than the control group (z = 2.803, P =0.005). However, there was no significant difference in pure Ti and Au-Pd groups compared with the control group (P＞0.05).

Pure Ti and Au-Pd alloy have a better biocompatibility than Co-Cr and Ti alloy in inflamed periodontal tissues.