To investigate the expression of LASP-1 in tongue squamous cell carcinoma, and to study the influence of shRNA silent LASP - 1 on cell proliferation and migration of tongue squamous cell carcinoma.

Green fluorescence shRNA targeting LASP- 1 was constructed and transfected into SCC9 cells by lipofectamine. Cell proliferation was detected by MTT method. LASP-1 mRNA and protein were determined by RT-PCR and western blot respectively. The migration ability of cells was detected by transwell assay.

There was green fluorescence expression in experimental and negative control group cells after transfecting shRNA and sh NC respectively. LASP - 1 mRNA and protein were decrease in experimental group cells. It indicated that LASP-1 shRNA was transfected successfully. By contrast with control group, the cell viability of experimental group cells reduced by (51.23 ± 1.47)% and (50.07 ± 2.11)% after 48 h and 72 h respectively. The results of transwell assay showed that migration ability of SCC9 cells was decreased significantly after shRNA targeting LASP-1 was successful transfected. It decreased by 43% compared with control group.

LASP-1 has high expression in tongue squamous cell carcinoma cells, down regulated LASP-1 gene expression can inhibit the proliferation and migration of SCC9 cells.