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Basic Study | Publishing Language: Chinese | Open Access

Influence of shRNA silent LASP-1 on cell proliferation and migration of tongue squamous cell carcinoma

Li-na YU1( ), Wen-xuan ZHANG, Yang YU, Wen-guang QIN, Xue-qi GUO
Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou 510140, China
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Abstract

Objective

To investigate the expression of LASP-1 in tongue squamous cell carcinoma, and to study the influence of shRNA silent LASP - 1 on cell proliferation and migration of tongue squamous cell carcinoma.

Methods

Green fluorescence shRNA targeting LASP- 1 was constructed and transfected into SCC9 cells by lipofectamine. Cell proliferation was detected by MTT method. LASP-1 mRNA and protein were determined by RT-PCR and western blot respectively. The migration ability of cells was detected by transwell assay.

Results

There was green fluorescence expression in experimental and negative control group cells after transfecting shRNA and sh NC respectively. LASP - 1 mRNA and protein were decrease in experimental group cells. It indicated that LASP-1 shRNA was transfected successfully. By contrast with control group, the cell viability of experimental group cells reduced by (51.23 ± 1.47)% and (50.07 ± 2.11)% after 48 h and 72 h respectively. The results of transwell assay showed that migration ability of SCC9 cells was decreased significantly after shRNA targeting LASP-1 was successful transfected. It decreased by 43% compared with control group.

Conclusion

LASP-1 has high expression in tongue squamous cell carcinoma cells, down regulated LASP-1 gene expression can inhibit the proliferation and migration of SCC9 cells.

Keywords

LASP-1shRNATongue squamous cell carcinomaCell proliferationMigration
CLC number: R739.86 Document code: A Article ID: 2096-1456(2016)06-336-05

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Journal of Prevention and Treatment for Stomatological Diseases
Volume 24 Issue 6,
June 2016
Pages 336-340
DOI: 10.12016/j.issn.2096-1456.2016.06.004

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YU L-n, ZHANG W-x, YU Y, et al. Influence of shRNA silent LASP-1 on cell proliferation and migration of tongue squamous cell carcinoma. Journal of Prevention and Treatment for Stomatological Diseases, 2016, 24(6): 336-340. https://doi.org/10.12016/j.issn.2096-1456.2016.06.004

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Received: 15 February 2016
Revised: 25 March 2016
Published: 20 June 2016
© 2016 by Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases

This article is licensed under a Creative Commons Attribution 4.0 International License.