Lactiplantibacillus plantarum MWFLp-182 was originally isolated from the feces of long-living elderly individuals in Hezhou city, Guangxi, China. L. plantarum MWFLp-182 showed favorable gastric and intestinal tolerance compared with Lactiplantibacillus rhamnosus GG (LGG). L. plantarum MWFLp-182 was further examined for its antioxidant and anti-inflammatory activity in ABAP-treated HT-29 cells. Importantly, it enhanced the expression of anti-inflammatory factor (interleukin-10 (IL-10)), antioxidant cytokines (superoxide dismutase (SOD), glutathione peroxidase (GPx), nuclear factor erythroid 2-related factor (Nrf2), and hemeoxygenase 1(HO-1)), B-cell lymphoma-2 (Bcl-2), and tight junction (Zonula occludens protein 1(ZO-1), Claudin-1, and Occludin) proteins and genes, and reduced reactive oxygen species (ROS). The complete genome of L. plantarum MWFLp-182 contained a single circular chromosome that was 3 257 196 bp long, with a G + C content of 44.49%, and a single circular plasmid that was 53 560 bp long. The genes tpx, trxA, trxB, npr, nrdH, dps, recA, gpx, gshA and arsC in this strain were related to antioxidant function; the key genes involved in antioxidant function were further investigated. In this study, we identified a probiotic candidate with antioxidant properties.

To investigate the effects of drinking the soaking of Malus domeri (Bois) Chev. leaves on gut microbiota and metabolites of long-living elderly individuals in Hezhou city, Guangxi, China. It has been reported that longevity is closely related to metabolism and the gut microbiota. The 16S rRNA sequencing and liquid chromatography-mass spectrometry (LC-MS) were used to analysis fecal samples and explore the factors affecting longevity in the region. Interestingly, we discovered, that elderly individuals who had been drinking the soaking of M. domeri (Bois) Chev. leaves for a long time exhibited higher diversity of the gut microbiota than without drinking the soaking, notably. The proportions of Ruminococcaceae and Prevotella were decreased in those who did not drink this soaking. In addition, a total of 106 metabolites were characterized, and the people of long-lived people (> 90 years old) and elderly people (< 90 years old) who drinking soaking of M. domeri (Bois) Chev. leaves signif icantly altered the gut microbiota and upregulate d levels of haplopine, farnesol, genipic acid, momordicinin, 2-hydroxyestrone, hydroxyphenyllactic acid, caffeic acid, sophoraflavanone B, and soyasaponin I. We preliminarily determined that M. domeri (Bois) Chev. leaves consumption may be an important factor affecting longevity in this area.