In order to explore the new technology of rice planthopper control based on cultivation and tillage measures and reduce the input of plant protection cost in agricultural production, four rice varieties with different resistance levels (Weiliangyou 7713 (Level 3), Hualiangyou 10 (Level 5), Fyou 498 (Level 9), Huiliangyou Yuenongsimiao (Level 9)) were used as materials to study the control effect of rice varieties with different resistance levels on rice planthopper by interspersing a layout resembling 回 (a Chinese character) and low toxicity pesticide control. The relative incidence of rice planthoppers in the whole growth period of the interspersed a layout resembling 回 (a Chinese character) was about 70%~97%. Compared with the conventional layout, the yield of rice was increased by 2.10%~4.28% under the condition of no pesticide application, and the lower the resistance level, the greater the yield increase. Under the condition of interspersed a layout resembling 回 (a Chinese character), compared with spraying treatment, the yield of rice without spraying treatment decreased by 2.31%~6.11%, and the lower the resistance level, the greater the yield reduction. The interspersed a layout resembling 回 (a Chinese character) combined with appropriate pesticide control had better control effect. Interspersing a layout resembling 回 (a Chinese character) using rice varieties with differential resistance on rice planthoppers could mitigate the damage caused by rice planthoppers and reduced the yield loss to a certain extent, but the specific effect was related to the resistance level of the varieties. The higher the resistance level of the peripheral varieties was, and the lower the resistance level of the central varieties was, the more the advantages of the interspersed a layout resembling 回 (a Chinese character) cultivation mode could be reflected.