To evaluate the efficacy and biosafety of a chloride ion-tolerant compound hydrogen peroxide disinfectant.

The efficacy of the compound hydrogen peroxide disinfectant was assessed using suspension quantitative bactericidal tests, inanimate surface disinfection tests, and air disinfection tests, respectively. Its biosafety was evaluated using animal toxicity test methods, including the acute oral toxicity test, single intact skin irritation test, and mouse bone marrow polychromatic erythrocyte micronucleus test.

At 20 ℃, the log reduction value (LRV) of the disinfectant diluted with the standard hard water exceeded 3.00 against four test microorganisms (E.coli, S.aureus, P.aeruginosa, and C.albicans) within 5 minutes. When sprayed onto wooden surfaces, the diluted disinfectant yielded an average LRV of over 1.00 against natural bacteria. The germicidal efficiency against S.albus was ≥99.90% in simulated air disinfection tests, and its bactericidal efficiency against airborne natural bacteria exceeded 90% after 30 minutes of exposure. The animal toxicity test results indicated that the compound hydrogen peroxide disinfectant was practically non-toxic, non-irritating to the skin, and negative in the mouse bone marrow micronucleus assay.

The disinfectant proves to be highly tolerant of chloride ions and quite safe, with rapid and broad-spectrum bactericidal efficacy after dilution with standard hard water.