Ovulation is paramount for female animal fertility, necessitating a thorough understanding of its process and molecular underpinnings. This study aimed to delineate the temporal dynamics of ovulation in the goat ovary. Utilizing single-cell sequencing, we analyzed follicular fluid samples obtained at 0, 6, 12, 18, and 24 h post-hCG administration, identifying 4 cell types and 6 myeloid cell subtypes. We elucidated gene expression and functional changes in granulosa cells (GCs) over the time course of ovulation. Notably, our study detected and confirmed immune cell infiltration at 6 h post-luteinizing hormone (LH) peak. Additionally, cell–cell communication analysis revealed strong predicted interactions between GCs and macrophages, involving signaling programs associated with immune-cell recruitment, extracellular-matrix remodeling, and oocyte maturation. Collectively, our investigation has established a comprehensive single-cell transcriptome atlas of the ovulatory goat follicular microenvironment for advancing exploration into ovulation mechanisms and developing therapies for ovulatory disorders.