With the advancement of biotechnology and computer technology, life sciences and medical data have grown rapidly, and research paradigms have undergone revolutionary changes. Biomedical and clinical research have entered the big data era, creating an urgent need to establish multi-dimensional, high-quality biobanks that effectively integrate multi-level clinical data and medical research data. This article introduces a large-scale, single-center, and informatized biobank established by Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology. This biobank integrates a large amount of medical data since 1980, encompassing multi-dimensional information with real-time updates. The biobank employs a four-tier data governance architecture to ensure standardized data acquisition, structured processing, and normalized management, enabling efficient data utilization while maintaining data security. The repository also collaborates closely with experts in management, biology, ethics, law, clinical medicine, and other fields to establish a comprehensive application and approval process, an ethical review and management framework, and a supervisory system to ensure compliant operation. Using intracerebral hemorrhage as an example, we demonstrate some research results obtained using this biobank resource, aiming to assist potential researchers. The Tongji Biobank is openly accessible to all researchers who wish to utilize this resource for health-related studies that benefit public health.