This paper studies a sampling-based dynamic event-triggered fixed-time bipartite formation algorithm for a class of continuous-time multi-agent systems with communication constraints. First, a periodic sampling mechanism is designed to reduce the system’s communication frequency. Then, a dynamic event-triggered control algorithm based on auxiliary variables is developed for sampled-data systems to further reduce the system’s triggering frequency. Next, to enhance the convergence speed of the dynamic event-triggered control method, a dynamic event-triggered fixed-time bipartite formation control scheme is investigated. Finally, using Lyapunov stability theory, signed graph theory, and relevant inequalities, a rigorous theoretical proof of the stability of the proposed control protocol is provided, and the algorithm’s effectiveness is verified through simulation experiments.