To investigate the ameliorative effects of polysaccharides from Ganoderma lucidum spore powder polysaccharide (GLSP) and fermented G. lucidum spore powder polysaccharide (FGLSP) on obesity in mice, providing an experimental basis for their development and application.

A mouse model of high-fat diet-induced obesity was established. After a 10-week intervention with high and low doses of GLSP and FGLSP, the effects of the two polysaccharides on body mass, body fat, glucose tolerance, insulin sensitivity, hyperlipidemia, liver function, hepatic oxidative stress, and pathological morphology of adipose and hepatic tissues were evaluated.

Compared with the model group, both GLSP and FGLSP interventions significantly reduced body mass, body fat, the area under the curve during oral glucose tolerance test (OGTT), fasting serum insulin levels, total cholesterol (TC) levels, serum leptin levels, liver mass, hepatic aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels and hepatic malondialdehyde (MDA) levels (P < 0.05). Additionally, they significantly increased serum adiponectin levels and catalase (CAT) activity (P < 0.05), and ameliorated pathological damage in adipose and hepatic tissues.

GLSP and FGLSP effectively ameliorated obesity in mice by reducing body mass gain and fat accumulation, ameliorating dyslipidemia and dysglycemia, and mitigating oxidative stress-induced hepatic injury.