For the full-scale fatigue test of a large amphibious aircraft, we conduct an in-depth analysis of its test characteristics, challenges, and requirements, and establishes an overall technical solution covering simulation of boundary conditions for the test article, load processing, construction of an integrated comprehensive test platform, development of test power systems, test control and measurement, as well as comprehensive monitoring throughout the entire test process. Innovative applications include follower constraint technology, load processing techniques based on load type decomposition, efficient and collaborative construction technology for an integrated comprehensive test platform, and multi-modal monitoring technology. Test results demonstrate that the implementation of these technologies achieves excellent outcomes, enabling precise simulation of complex load conditions for the aircraft. This provides a reliable technical foundation for verifying the structural durability and damage tolerance of amphibious aircraft. The technical achievements hold significant reference value for subsequent full-scale fatigue testing.