Seeds of major Populus cultivars were collected from across China in 2024 to build the image-data bank of over 1187000 images of singular seeds for the National Forestry and Grassland Science Data Center (NFGSDC). An innovative vibration-assisted machine-vision system was built with alternating back-lit and front-lit illumination, which incorporated a flexible vibratory panel (FVP) to manipulate the multitude of seeds to minimize the occurrence of butting or overlapping, and the lighting from alternating directions to capture phenotypic features both in silhouettes and in vivid color images. To investigate how illumination directions would affect phenotyping, morphological and chromatic metrics were measured, respectively from only the common front-lit images and through the combined use with back-lit images, and applied to distinguish different cultivars and harvest-batches. Results verified that back-lit excelled for reliable segmentation for feature images and accurate morphological metrics, especially when the closeness was clearly revealed in the clustering dendrogram between Nanlin 895 and Zhonglin 46, which shared a common genetic sourcing from P. Euramericana. In contrast, front-lit images were prone to occasional segmentation defects leading to inaccurate morphological measurements due to the highly dynamic range of seed colors, which caused the clustering to lose the genetic relevance. The power of the image-dataset of alternating illuminations was further demonstrated when a decent accuracy of 0.819 yielded from the simple support-vector-machine classification while working on only the back-lit morphological measurements, and the increase to 0.856 with statistical significance if with the addition of chromatic metrics from corresponding front-lit color images, while other image characteristics had been strictly held back. The vibration-assisted alternating illumination protocol established in this work to capture delicate seed-features of Populus cultivars may also be applied to other small grains facing similar imaging challenges, laying a sturdy step-stone of high-throughput phenotyping for large-scale breeding programs and genetic studies.
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The quality assessment of agricultural and forestry products is a core process in ensuring food safety and enhancing product competitiveness. Traditional detection methods suffer from drawbacks such as sample destruction, expensive equipment, and poor adaptability. As an innovative analytical technique combining optical and acoustic detection principles, photoacoustic spectroscopy technology (PAS) overcomes the limitations of conventional detection techniques that rely on transmitted or reflected optical signals through its unique light-thermal-acoustic energy conversion mechanism. With its non-contact, high-sensitivity, and multi-form adaptability characteristics, PAS has been increasingly applied in the quality assessment of agricultural and forestry products in recent years, providing a new solution for the simultaneous detection of internal and external quality in these products.
In the specific applications of agricultural and forestry product testing, PAS has demonstrated practical value in multiple aspects. In seed testing, researchers have established quantitative relationship models between photoacoustic signals and seed viability also achieved dynamic assessment of seed health by monitoring respiratory metabolic gases (e.g., CO2 and ethylene). In fruit and vegetable quality analysis, PAS can capture characteristic substance changes during ripening. In the quality control of grain and oil products, Fourier-transform infrared PAS technology has been successfully applied to the rapid detection of protein content in wheat flour and aflatoxin in corn. In food safety monitoring, PAS has achieved breakthrough progress in heavy metal residue detection, pesticide residue analysis, and food authenticity identification.
Despite its evident advantages, PAS technology still faces multiple challenges in practical implementation. Technically, the complex matrix of agricultural and forestry products causes non-uniform generation and propagation of photoacoustic signals, complicating data analysis. And environmental noise interference (e.g., mechanical vibrations, temperature fluctuations) compromises detection stability, while spectral peak overlap in multi-component systems limits quantitative analysis accuracy. Equipment-wise, current PAS systems remain bulky and costly, primarily due to reliance on imported core components like high-power lasers and precision lock-in amplifiers, severely hindering widespread adoption. Moreover, the absence of standardized photoacoustic databases and universal analytical models restricts the technology's adaptability across diverse agricultural products. Looking forward, PAS development may focus on these key directions. Firstly, multi-technology integration by combining with Raman spectroscopy, near-infrared spectroscopy, and other sensing methods to construct multidimensional data spaces for enhanced detection specificity. Moreover, miniaturization through developing chip-based detectors via micro-electromechanical technology, replacing conventional solid-state lasers with vertical-cavity surface-emitting lasers (VCSELs), and adopting 3D printing for integrated photoacoustic cell fabrication to significantly reduce system size and cost. Furthermore, intelligent algorithm innovation with incorporating advanced deep learning models like attention mechanisms and transfer learning to improve interpretation of complex photoacoustic spectra. As these technical bottlenecks are progressively overcome, PAS is poised to establish a quality monitoring network spanning the entire "field-to-market" chain—from harvesting to processing/storage to distribution —thereby transforming agricultural quality control from traditional sampling-based methods to intelligent, standardized, full-process monitoring. This will provide technical support for food safety assurance and agricultural industry advancement.
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