To investigate the clinical manifestations of traditional Chinese medicine (TCM) and their associations with TCM constitutions in individuals who have migrated to plateau areas, and to provide a scientific basis for plateau health management.

Migrants living in areas above 3000 m were selected as research subjects. Data were collected by using TCM symptom assessment scales and constitution assessment scales. Descriptive statistical analysis was conducted to determine the incidence and severity of symptoms among individuals with different migration durations, and core symptoms were identified. Factor analysis was performed by using SPSS software to extract symptom clusters and explore the correlation between core symptoms and TCM constitutions.

Among individuals who migrated to plateau areas, the incidence of discomfort symptoms was 83.44%. The five most common symptoms were dry skin (67.94%), forgetfulness (56.03%), dry mouth (52.06%), yellow urine (48.73%), and insomnia (47.14%). In the top 10 symptoms with the highest increase in incidence, yellow urine (33.51%) and forgetfulness (26.33%) were both present in the top 10 symptoms across different migration durations. Factor analysis extracted 5, 2, 4, and 6 symptom clusters from the overall population, individuals who migrated within 1 year, those who migrated for 1-2 years, and those who migrated over 2 years, respectively. Qi-deficiency constitution (QDC), blood stasis constitution (BSC), qi stagnation constitution (QSC), phlegm-dampness constitution (PDC), and dampness-heat constitution (DHC) were significantly positively correlated with forgetfulness.

Migrating to plateau areas can induce discomfort symptoms, and both the number and incidence of symptoms increase with longer migration durations. The number and incidence of high-frequency symptoms (incidence≥30%) increase with prolonged migration time. There are differences in the composition and severity of symptom clusters across different migration durations. QDC, BSC, QSC, PDC, and DHC are closely related to forgetfulness and can be considered risk constitutions for forgetfulness. Timely attention to changes in symptom clusters and constitutions can help prevent and mitigate the occurrence and development of symptoms.

Infected wounds caused by bacteria such as Escherichia coli and Staphylococcus aureus pose significant challenges during the healing process. Hydrogels have emerged as promising materials for the treatment of such infections, as they have the potential to deliver therapeutic agents while supporting tissue repair. This study aimed to develop ε-PLL@SA/Gel (PSG) hydrogels by incorporating varying concentrations of ε-poly-L-lysine (ε-PLL) into sodium alginate/gelatin (SA/Gel) using calcium chloride as a crosslinking agent, and to evaluate their antibacterial efficacy.

The mechanical properties, biocompatibility, antibacterial activity of hydrogels were evaluated. Biocompatibility was examined by measuring cell viability and proliferation of human skin fibroblasts in vitro. Antibacterial efficacy against Escherichia coli and Staphylococcus aureus was quantified using bacterial inhibition assays. The wound healing efficacy of the hydrogels were evaluated in mouse models of infected wounds.

PSG hydrogels exhibited excellent mechanical strength, injectability, and self-adhesive properties. In vitro, hydrogel treatment resulted in high cell viability and promoted human skin fibroblast proliferation. PSG15 exhibited the highest antibacterial activity and inhibited E. coli and S. aureus by 89.53% and 92.21%, respectively. In vivo, PSG15 significantly accelerated wound healing, enhanced angiogenesis, and regulated macrophage polarization by increasing CD206 expression and decreasing CD80 expression. Additionally, PSG15 modulated the skin microbiota, reduced pathogenic bacterial abundance and maintained microbiota diversity.

The PSG15 hydrogel is a promising candidate for the treatment of infected wounds because it inhibits bacterial growth, promotes tissue repair, and modulates the wound microbiota.