To study the vascularization of the collagen-binding basic fibroblast growth factor (bFGF)-loaded collagen membranes in soft tissue repair in the hard palates of rats.

Ninety-six male 6-week-old Wistar rats were randomly divided into 4 groups, and 3-mm-diameter circular soft tissue defects were produced from the distal surface of the third molar to the mesial surface of the first molar in their hard palates. The defects were covered with a collagen-targeting bFGF/collagen membrane, a free bFGF/collagen membrane, a collagen membrane or no membrane (control group). Every 6th rat was randomly sacrificed at 1, 2, 4 and 8 weeks in every group after surgery. Wound healing and the number of new blood vessels were measured by hematoxylin-eosin (HE) staining.

The numbers of new blood vessels in the collagen-targeting bFGF/collagen membrane group were 8.94 ± 0.61, 17.39 ± 2.08 and 11.22 ± 1.66 at 1, 2 and 4 weeks after surgery, respectively, which were significantly greater than the values in the other groups (P<0.05). At 8 weeks, the number of new blood vessels in the collagen-targeting bFGF/collagen membrane group was 4.17 ± 1.28, and there was no significant difference in the numbers of new blood vessels between any of the groups (P > 0.05).

Collagen-targeting bFGF/collagen membranes had a favorable effect on promoting angiogenesis during wound healing, and promoted wound healing.

To investigate the effect of pure titanium surface of large diameter TiO₂ nanotubes modified by RGD peptide on the adhesion and proliferation of MG63 osteoblasts. to provide theoretical proof for developing titanium implants.

Commercially pure titanium discs were divided into four groups and treated with SLA to obtain a microrough surface (SLA group). Then, nanotubes were imposed on this microrough surface by anodization (SLA+ 80 group). The surface was then modified by dopamine (DOPA) (DOPA Group), after which bioactive RGD peptide layers were generated on the TiO₂ nanotube surfaces via electrochemical and molecular self-assembly techniques (RGD group). The titanium surface morphology and elemental composition of each group were characterized by field emission scanning electron microscopy (FE-SEM) and X-ray photoelectron spectroscopy (XPS). MG63 cells were cultured in vitro to evaluate biological activities of titanium before and after treatment, including the evaluation of early-stage cell adhesion capacity by fluorescence microscopy, proliferation capacity by MTS assay, and mRNA expression of the cell osteoblast-related genes alkaline phosphatase (ALP) and osteocalcin (OCN) by qRT-PCR.

FE-SEM and XPS showed that hierarchical micro/nanosurfaces decorated with TiO₂ nanotubes were produced on titanium using sandblasting and large grit etching combined with anodization, dopamine was then self-polymerized to form a polydopamine film on the TiO₂ nanotube surfaces, and RGD peptides were then conjugated to the polydopamine film, finally forming RGD peptide-modified bioactive layers. In vitro experiments showed that compared with the other three materials, the RGD-modified material was more conducive to cell adhesion and proliferation (P < 0.05). The expression levels of ALP and OCN mRNA in the RGD group were significantly higher than those in the SLA group and DOPA group (P < 0.05).

Hierarchical micro/nanosurfaces decorated with TiO₂ nanotubes functionally modified with RGD peptides have good biocompatibility and could be used for developing titanium implants and further improving early osseointegration.

To explore the clinical effect of the alveolar crest approach in the treatment of odontogenic maxillary sinusitis and the repair of edentulous implants in this area.

This was a retrospective case series of 20 patients with odontogenic sinusitis. The pathogenesis in each case was investigated. After elimination of the dental origin, each patient was treated with flushing, drainage and anti-inflammatories through the alveolar crest approach. Postoperative CBCT reexamination was performed to evaluate the therapeutic effect. Maxillary sinus elevation surgery with simultaneous or delayed implantation was performed after maxillary sinusitis healing was confirmed. The patients were followed postoperatively.

Twenty patients with odontogenic sinusitis were treated by the alveolar crest approach, and 17 were cured, for a cure rate of 85%. Among them, 17 of the maxillary sinusitis patients were followed for 1 year, with good results.

The alveolar crest approach is feasible for the treatment of odontogenic maxillary sinusitis and can serve as a minimally invasive method for the repair of edentulism in this area and implantation.