To investigate the changes of vaspin and TNF-α levels in gingival crevicular fluid (GCF) of type 2 diabetic (T2DM) patients with chronic periodontitis (CP) after non-surgical periodontal treatment.

60 subjects were divided into 4 groups: DM-CP group (patients with both T2DM and CP, n＝15); CP group (CP patients without T2DM, n＝15); DM group (T2DM patients without CP, n＝15), and CTRL group (systemically and periodontally healthy individuals, n＝15). The clinical parameters of periodontal tissue and GCF were measured before and 8 weeks after non-surgical periodontal treatment. The levels of vaspin and TNF-α were measured by ELISA.

The levels of vaspin and TNF-α in CP group were significantly higher than those in CTRL group (P < 0.05), while the levels of vaspin and TNF-α in CP group were significantly decreased after treatment (P < 0.05). There was a statistically significant positive correlation between the total amount of vaspin and the total amount of TNF-α, the level of HbA1c, gingival index (GI) and probing depth (PD) (P < 0.05).

The results shows that vaspin and TNF- α are greatly decreased in periodontitis after non-surgical periodontal treatment. It suggests that vaspin and TNF- α in GCF may serve as inflammatory markers for the diagnosis and prognosis of diabetes and periodontitis.