To evaluate the effect of Wnt inhibitor XAV-939 on the proliferation and adipogenic differentiation of dental pulp stem cells (DPSCs).

The effect of XAV-939 on the proliferation of DPSCs was analyzed by cell counting kit-8(CCK-8). The effect of XAV-939 on the adipogenic differentiation of DPSCs was analyzed by oil red staining. Quantificational real-time polymerase chain reaction (qRT-PCR) was used to analyze the gene expression of glycogen synthase kinase-3β(GSK3β) and β-catenin which were Wnt signaling marker genes, and peroxisome proliferator activated receptor-γ2 (PPARγ2) and CCAAT/enhancer binding protein α(C/EBPα) which were adipogenic marker genes.

After 14 days, XAV-939 up-regulates expression of GSK3β、PPARγ2 and C/EBPα, while down-regulates β-catenin.

XAV-939 could promote the adipogenic differentiation of DPSCs by inhibiting Wnt signalling pathway.