Objective Exploring the effect of three-flap paltoplasty in preventing anterior palatal fistula for patients whose anterior fissures measured more than 0.5 cm.

12 patients aged 18-24 months with unilateral complete cleft palate were selected for the implementation of three-flap paltoplasty for cleft palate repair. Briefly, three-flap paltoplasty is based on the traditional two-flap paltoplasty method and involves the creation of a mucoperiosteal flap A in the contralateral palate in front of the fissure margin that is approximately half the size of the anterior palate. The flap A was sutured to the edge of the contralateral nasal mucosa, and the mucoperior flap of both sides of the loose fissure was sutured in layers, and the suture was removed two weeks after surgery. The recovery of cleft palate was observed.

All patients were followed up for 3 months, and 12 patients underwent successful repairs with no fistula and other complications.

Three-flap paltoplasty is an effective method of preventing anterior palatal fistula.

To explore the role of Wnt signaling pathway in the process of epithelial layer's disappearance when Oxydiethanoldiethylene GlcNAc influence development of upper lip.

The over/lower-expression of O-GlcNAc and reverse-high expression experimental group was constructed by transplanting beads into the upper lip of chicken embryo which contain O-GlcNAc promoter OGT (O-GlcNAc transferase), O-GlcNAc inhibitor OGA (O-GlcNAcase) or OGA inhibitor STZ (streptozoticin). and kept incubation for 24 h. Phenotypic analysis of skeletal and morphological changes were performed by using Alizarin Red/Alcian Blue method. TUNEL assay was performed to analyze the cell apoptosis. Immunohistochemistry method was performed to test the Wnt signaling pathway including GSK-3β, β-catenin.

In the group OGZ and STZ where O-GlcNAc was overexpressed, disappearance of the epithelial layers was delayed, embryo lips appeared bone defect in bonestaining, compared with the normal group, obvious cell apoptosis has not been found in TUNEL detection. But expression of GSK-3 beta increased in the cytoplasm, expression of beta-catenin nucleus reduced, upper lip development of normal side and the low expression of O-GlcNAc in the OGA group were not affected.

Overexpression of O-GlcNAc may lead to a delay of epithelial disappearance which was not caused by the apoptosis but maybe caused by inhibiting Wnt signaling pathway. The process can change related signaling factors including GSK-3 beta, beta-catenin, which may influence the development of upper lip and in the end lead to cleft lip and palate.

To discuss the demand and security of tooth extraction under general anesthesia with endotracheal intubation for the special needs patients.

From January 2014 to December 2015, the cases of tooth extraction under general anesthesia were collected to analyse the demand and security of tooth extraction with general anesthesia.

54 patients were recruited in the study in which 11 were with serious limitation of mouth opening, 10 were with serious cardiovascular disease risk, 2 were with history of epilepsy, 13 were with serious dental phobia, and 18 were incoordination patients. All the operations were performed successfully and safely, and all the scores of post anesthetic discharge scoring system exceeded 9 points. No developed complications were showed in 1 day, 1 week, 1 month follow-up.

Tooth extraction under general anesthesia with endotracheal intubation is a safe way for special needs patients.

To evaluate the accuracy of cone beam CT (CBCT) in calculating the volume of the bone defect in alveolar cleft.

14 patients with unilateral alveolar cleft were included in this study. Their image data were collected by CBCT scan and imported in the Mimics 10. 01 software. The volume of the bone defect in alveolar cleft was calculated by Mimics 10. 01 software. The real bone volumes transplanted during operation were measured by sterile syringes and were compared with preoperative CBCT data.

The volume of bone defect from CBCT calculation was (2. 78 ± 0. 70) mL, while the real volume obtained in operation was (2. 88 ± 0. 70) mL. There was no significant difference between the CBCT data and the real bone defect (P ＞ 0. 05). The accuracy of CBCT in predicting bone defect volume was (95. 09 ± 2. 56) %.

The combination of CBCT data and Mimics software can evaluate the bone defect volume in alveolar cleft accurately.