With the increasing demand for sustainable foods among consumers, plant-based meat, as an alternative to traditional meat, has received increasing attention. High-moisture extrusion is one of the crucial steps in the preparation of plant-based meat, which utilizes the synergistic effects of high temperature, high pressure, and high shear to mimic the fibrous structure and texture of meat. However, this process leads to complex physical and chemical changes in proteins and auxiliary materials, resulting in significant differences between the product and traditional meat in terms of color, texture, and flavor. Therefore, the addition of exogenous additives has become a key research area for improving product quality. This paper reviews the effects of different additives (such as proteins, lipids, polysaccharides, crosslinking agents, and other additives) on the structure and quality of plant-based meat during high-moisture extrusion, explores the mechanisms of protein-protein, lipid-protein, polysaccharide-protein, and crosslinking agent-protein interactions, and analyzes their enhancing effects on the texture, flavor, and nutritional value of plant-based meat. This paper aims to provide a theoretical basis and technical support for further research and development of plant-based meat products.
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Open Access
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Open Access
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To investigate the regulatory effect of equol (Eq) on the susceptibility to metabolic syndrome (MS) in the offspring of rats with gestational diabetes mellitus (GDM).
Twenty female SD rats were divided into two groups: the normal group, which was fed a normal diet, and the GDM group, which was fed a high-fat diet (HFD) combined with intraperitoneal injection of streptozotocin. Each female rat was caged with a male to establish a normal pregnancy group and a GDM pregnancy group. The male offspring from the normal pregnancy group were randomly divided into two subgroups: blank control and MS model control. The male offspring from the GDM pregnancy group were randomly divided into five subgroups: MS model, low-, medium- and high-dose Eq (intragastric administration of 20, 40 and 80 mg/kg of Eq, respectively) and positive control (5 mg/kg of simvastatin). The blank control group was fed a normal diet, and the other groups were fed a HFD, with 8 rats in each group. The serum glucose and lipid metabolism indexes, oxidative stress and inflammation levels of GDM offspring rats were detected by commercial kits, and the degree of liver steatosis was observed by hematoxylin-eosin (HE) staining.
Compared with the MS group, Eq reduced the Lee’s index, final body mass and liver index of GDM offspring rats, and high-dose Eq decreased the Lee’s index by 13.9% (P < 0.01). Meanwhile, high-dose Eq significantly reduced serum triglyceride (TG), total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) levels (P < 0.05), highly significantly increased serum high-density lipoprotein cholesterol (HDL-C) levels (P < 0.01), and significantly reduced serum fasting blood glucose (FBG) and fasting insulin (FINS) levels (P < 0.05). Furthermore, it highly significantly lowered serum malondialdehyde (MDA), interleukin-6 (IL-6) and tumor necrosis factor-α(TNF-α) levels and increased serum glutathione peroxidase (GSH-Px) levels (P < 0.01).
GDM offspring are more susceptible to MS than the offspring of normal pregnant rats. Eq can significantly improve abnormal glucose and lipid metabolism in GDM offspring, enhance antioxidant capacity, alleviate inflammation, and reduce MS in multiple ways.
Open Access
Review
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Plant proteins, as natural amphiphilic macromolecules, are mainly derived from plants such as soybean and pea. Due to their excellent functional properties such as emulsification, foaming and gelling, they have significant potential and research value in various fields such as the food industry, biomedicine, and material science. However, emulsions formed solely from proteins are prone to instability due to the influence of various environmental factors. Existing studies have shown that the introduction of polysaccharides can effectively improve the interfacial structure and performance of plant protein-stabilized emulsions. As a common natural polysaccharide, cellulose has attracted widespread attention due to its good biocompatibility, biodegradability and low cost. Based on covalent and non-covalent interactions, plant protein-cellulose stabilized emulsions exhibit stable network structures and excellent properties. To provide a better understanding of recent progress in plant protein-cellulose stabilized emulsions, this paper reviews the raw materials, preparation methods and emulsion properties, focusing on the factors influencing the structures and properties of plant protein-cellulose stabilized emulsions. Meanwhile, it summarizes the current status the application of these emulsions, aiming to provide insights for the improvement and utilization of protein-cellulose stabilized emulsions.
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