The effects of the addition of different amounts (0.01%, 0.05% and 0.10%) of banana flower extract (BFE) on the physicochemical quality of fermented yak meat sausage were investigated using butylated hydroxy toluene (BHT) as a positive control, and the changes in the pH, moisture content, color, water retention, texture, rheological properties, total bacterial count, volatile flavor substances and sensory quality of the sausage during fermentation and storage were evaluated. The results showed that the sausage with 0.10% BFE had stronger water retention and bacterial inhibition ability, while increasing BFE addition reduced the textural characteristics and storage modulus of the fermented sausage. A total of seven classes of volatile flavor substances, including alcohols, aldehydes, acids and esters, were detected in all samples during fermentation, and three principal components were obtained by principal component analysis, with a cumulative contribution of 75.014%. In conclusion, BFE has strong antioxidant activity and significantly improves the physicochemical properties of fermented yak meat sausage, and the most pronounced effect is obtained with 0.01% BFE addition.

The effects of adding different levels (0.01%, 0.05% and 0.10%) of banana flower extract (BFE) versus dibutylated hydroxy toluene (BHT) on protein oxidation in naturally fermented yak meat sausages were investigated. lys The carbonyl content, Schiff base content, total sulfhydryl content, active sulfhydryl content, surface hydrophobicity, dimeric tyrosine content, endogenous fluorescence intensity and solubility of myofibrillar proteins (MPs) from fermented yak meat sausage were measured at different fermentation times, and the secondary structure and composition of MPs were characterized by Fourier transform infrared (FTIR) spectroscopy, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The results showed that compared with the blank control, the addition of BFE decreased the carbonyl group content, Schiff base content, surface hydrophobicity, dimeric tyrosine content and endogenous tryptophan fluorescence intensity of MPs, and increased the total sulfhydryl group content, active sulfhydryl group content and solubility. FTIR spectroscopy showed that the addition of BFE improved the reveal the more stability of the secondary structure of MPs. SDS-PAGE showed that BFE reduced the diffusion of myosin heavy chain and had a protective effect on myosin. This study showed that BFE inhibited protein oxidation in naturally fermented yak sausage, and this effect was positively correlated with BFE concentration, being most pronounced at 0.10% BFE concentration.