A new dual-template molecularly imprinted polymer (MIPs) for efficient adsorption of triazines was prepared with cyromazine and melamine as template molecules, and its adsorption performance was evaluated for triazine residues in fruits and vegetables. Under the optimized condition of molar ratio of cyromazine to melamine = 3:2, the highest adsorption capacity of MIPs was achieved. The maximum adsorption capacity of MIPs at two specific binding sites 2.85 and 5.46 mg/g, and the binding constants were 26.688 and 80.775 mg/L, respectively. The adsorption process attained equilibrium in 100 minutes and showed a higher adsorption capacity than did non-imprinted polymers (NIPs). Molecular imprinting-based solid-phase extraction (MI-SPE) combined with high performance liquid chromatography (HPLC) was used for the measurement of four triazine residues including atrazine, prometryn, ametryn, and simetryn in cucumber, apple, and corn. The limit of detection of this method was 0.01–0.03 ng/mL, and the average recoveries of spiked samples at concentrations ranging from 0.1 to 0.5 μg/mL were 83.2%–102.3% (n = 3), with relative standard deviation (RSD) between 2.3% and 6.8%. The proposed method, therefore, is suitable for the trace detection of triazine residues in fruit and vegetable samples.
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Open Access
Review
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The gastrointestinal tract is the largest organ in the human body that communicates with the outside world. It has the functions of digesting food and transporting and absorbing nutrients. The gastrointestinal mucosal barrier secures gastrointestinal function through physical, chemical, biological, and immunologic barriers, and its damage can lead to chronic inflammation or acute and chronic diseases. Polysaccharides are one of the major bioactive substances in edible and medicinal mushroom and can be used as an auxiliary treatment for a variety of gastrointestinal diseases. This article reviews the roles of polysaccharides from edible and medicinal mushroom in regulating gastric health such as eliminating gastric Helicobacter pylori, protecting against gastric mucosal damage and gastric ulcer, and fighting against gastric cancer, summarizes the roles of polysaccharides from edible and medicinal mushroom in maintaining intestinal homeostasis and regulating the intestinal microbiota, alleviating inflammatory bowel disease and inhibiting colon cancer, and analyzes the regulatory mechanisms of polysaccharides from edible and medicinal mushroom on gastrointestinal function. We expect that this review will provide a reference for in-depth research on the promoting effect and mechanism of polysaccharides from edible and medicinal mushroom on gastrointestinal health.
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