Scholar - SciOpen

To investigate the effect of brain and muscle arant-like-1 (Bmal1) and miRNA-155-5p on the proliferation ability and aging of bone marrow mesenchymal stem cells (BMMSCs) to provide an experimental basis for elucidating the mechanism of bone senescence.

Methods

BMMSCs were extracted from the femur medullary cavity of 1-month-old mice, purified and cultured via the whole bone marrow mesenchymal adherent method and passed to P3. The characteristics of BMMSCs were detected by flow cytometry. BMMSCs were transfected with lentivirus to construct stable miR-155-5p and Bmal1 overexpression/interference BMMSCs. shRNA-transfected BMMSCs were identified by qRT-PCR. The proliferation activities of miR-155-5p and Bmal1 overexpression/interference BMMSCs were detected via CCK-8 assay. The apoptosis rates were measured by flow cytometry. The aging status of BMMSCs was identified with the senescence-associated β-galactosidase (SA-β-Gal) test. The expression of senescence-related genes P16 and P53 was detected by qRT-PCR.

Results

The shRNA-transfected BMMSCs were successfully generated. The proliferation ability decreased, and the apoptosis rates, the activity of SA-β-Gal and the relative expression levels of P53 and P16 increased when miRNA-155-5p was overexpressed. The proliferation ability increased, and the apoptosis rates, the activity of SA-β-Gal and the relative expression levels of P53 and P16 decreased when miRNA-155-5p was inhibited. The effect of Bmal1 is opposite to that of miRNA-155-5p.

Conclusions

The expression of Bmal1 promotes the proliferation and antiaging ability of BMMSCs, while miRNA-155-5p inhibits the proliferation and accelerates the aging of BMMSCs.

Open Access Review Article Issue

The anatomical limit and strategies for molar movement during orthodontic treatment

Yuqing YANG, Qing ZHAO

Journal of Prevention and Treatment for Stomatological Diseases 2023, 31(9): 667-672

Published: 20 September 2023

Abstract

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In many cases, tooth movement over a considerable distance is needed to meet the major goal of orthodontic treatment, which has always been to correct malocclusion and improve the facial profile in patients with skeletal malocclusion. However, tooth movement over a considerable distance also carries risks of dehiscence, fenestration, root exposure, and so forth. The reason lies in neglecting many limits for tooth movement, especially anatomical characteristics. This review focuses on structural limits for orthodontic molar movement, such as the alveolar cortex, the maxillary sinus floor, and the mandibular canal. In addition, we set the strategy in clinical orthodontics. For the alveolar cortex and the mandibular canal, orthodontists are recommended to move the root away from the cortical bone initially and formulate personalized molar movement plans according to clinical examination and cone-beam computed tomography (CBCT) and other imaging examinations. First, the molar root was controlled by torque away from the bone plate, and then, the molar movement amount and direction were controlled according to the personalized movement path. In regard to the maxillary sinus floor, light and continuous forces and scientific biomechanics are suitable for bodily tooth movement. In summary, better therapeutic efficacy and long-term stabilization could be achieved by circumventing the limits and risks caused by anatomical limitations and characteristics.

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