Potato is a vital global food source, yet the metabolic and transcriptional regulation governing tuber development and quality remains poorly understood. Here, we performed integrated metabolomic and transcriptomic analyses in wild and cultivated potato tubers, revealing dynamic and distinct metabolic accumulation patterns. The 849 metabolites exhibited 10 distinct temporal accumulation patterns, including six shared patterns between accessions and four genotype-specific patterns. The wild genotype exhibited an early decrease in lipids, followed by an increase in phenolic acids, whereas the cultivated genotype displayed an early increase in phenolic acids, accompanied by a decrease in some phenolic acids, amino acids, and flavonoids. A comparative analysis highlighted the cultivated genotype exhibited significantly lower levels of bitter steroidal glycoalkaloids (SGAs) but higher levels of beneficial flavonoids compared with its wild relative. Co-expression network analysis revealed 35 SGA-related and 57 phenylpropanoid-related genes that underlie metabolite dynamics. Notably, we functionally validated that the transcription factor StMYB113 plays a previously unknown role in positively regulating phenolic acid biosynthesis in tuber flesh. Our work provides a comprehensive map of tuber metabolism and a valuable resource for accelerating the genetic improvement of key potato quality traits.

Potato (Solanum tuberosum) is a globally important staple crop. However, cultivated potato varieties are highly sensitive to low temperatures. The molecular mechanisms underlying freezing resistance in potatoes remain poorly understood. Through comparative metabolome and transcriptome analyses of freezing-tolerant (CM, Solanum commersonii) and freezing-sensitive (DM, DM1-3516R44) varieties, we identified a cold-induced UDP-glycosyltransferase gene, ScUGT73B4, which is associated with the accumulation of glycosylated flavonoids in wild CM varieties. Overexpression of ScUGT73B4 led to increased accumulation of glycosylated flavonoids and enhanced antioxidant capacity, resulting in improved freezing tolerance in potato plantlets. These findings reveal a UDP-glycosyltransferase in the flavonoid pathway and offer a potential valuable genetic resource for breeding potatoes with improved freezing tolerance.