To investigate the effects of platycoside G1 (PG1) on senescent cells, it was determined that PG1 exerts a specific inhibitory effect on the senescent phenotype of hepatocytes by employing β-galactosidase staining and the cell counting kit-8 (CCK-8) assay. Flow cytometry, immunofluorescence, and Western blot indicated that PG1 inhibited cellular senescence primarily through its regulatory effects on autophagy. Furthermore, XFe96 metabolic flux analysis revealed that PG1 significantly enhanced aerobic glycolysis while concurrently inhibiting mitochondrial metabolism. In conclusion, PG1 specifically inhibits cellular senescence. This study provides both experimental and theoretical foundations for the development of PG1-based anti-aging therapeutics.