The aging of the population is one of the most concerns in the medical circle. Exploring the safe and effective anti-aging protocol is important in the study. The paper summarized the relevant articles for the anti-aging effect mechanism of acupuncture and moxibustion in recent 10 years from 9 aspects such as resisting oxidation and free radical damage, and regulating body immunity, neuroendocrine function, cell autophagy, aging-related gene expression, the deacetylase (Sirtuins) family, telomere with telomerase, epigenetic and iron metabolism. It is pointed out that acupuncture-moxibustion refers to the holistic therapy for anti-aging, characterized as multiple targets, multiple channels and full dimensions. The multidisciplinary cross-over study should be conduced on the basis of the dual regulatory effect of acupuncture and moxibustion, combined with modern life science and artificial intelligence technology, which is significant to reveal the scientific mechanism of acupuncture and moxibustion for anti-aging.