Infectious diseases such as coronavirus disease 2019 (COVID-19) continue to pose significant global health challenges. Effective management of reinfection risks depends on sustained levels of binding and neutralizing antibodies. However, conventional methods—such as enzyme-linked immunosorbent assays (ELISA) and virus neutralization tests (VNT)—are limited by complex workflows, long assay durations, and high sample volume requirements, making them less suitable for routine, decentralized, or time-sensitive surveillance. This study presents a custom-developed tip optofluidic immunoassay (TOI) platform that enables rapid, multiplexed antibody profiling using only 1 μL of fingertip blood. The system integrates batch-fabricated microfluidic immunoreactors with a portable chemiluminescent imaging station, completing both binding and neutralization capability assessments within 40 min. TOI achieves a broad dynamic range (3–4 orders of magnitude), high signal-to-noise ratio (~10,000), and excellent sensitivity for immunoglobulin G (IgG) detection. A renovated version of the rapid in vitro inhibition assay (RIVIA) is incorporated to evaluate neutralizing antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) with greater speed and cost-efficiency. In clinical studies, TOI successfully quantified antibody protection against multiple variants, identifying individuals with broad-spectrum immunity to both wild-type and XBB strains. With its high-precision, rapid turnaround, and minimal sample requirement, TOI offers a valuable tool for decentralized immune surveillance and personalized immunization strategy development.

Nanomanipulation based on atomic force microscopy (AFM) is widely used in various fields, including nanoparticle assembly, nanostructure construction, and semiconductor device manufacturing. However, a challenge remains in the lack of nanoscale visual feedback, which limits operational efficiency and accuracy. Here, we present a method for nanomanipulation under the visual guidance of real-time super-resolution imaging. The method involves coupling a microlens to the end of a conventional AFM probe cantilever and depositing a diamond tip onto the surface of the microlens using focused ion beam (FIB). The resulting microlens-AFM probe enhances the imaging resolution of traditional AFM optical systems, providing super-resolution capability with a multiple 3× increase in optical imaging magnification and enabling synchronous imaging and manipulation of silver nanowires with a characteristic size of 200 nm. This advancement bridges the key gap in AFM-based nanomanipulation by providing in-situ, real-time, non-destructive, and ultra-high-resolution visual feedback. This technology has the potential to provide new methods and key technologies for research in a wide range of applications, including nanorobots, nanoobservation, nanomanipulation, and manufacturing.