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Effects of Different Types of Fertilizers and Nitrogen Levels on Nitrogen Utilization, Yield and Quality of Weak Gluten Wheat
Scientia Agricultura Sinica 2025, 58(18): 3690-3709
Published: 16 September 2025
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【Objective】

This research investigated the effects of different types of fertilizers on the field traits, nitrogen absorption and utilization, yield, yield components, and processing quality of weak-gluten wheat following rice cultivation in the Jiang-Huai region. The goal was to provide a theoretical basis for the selection of optimal fertilizer types and regulation of application rates for achieving high yield and quality of weak-gluten wheat in this region.

【Method】

The two weak-gluten wheat varieties, including Yangmai 20 (YM20) and Baihumai 1 (BHM1), were used as experimental materials. The experiment was set three nitrogen levels: 150 kg·hm-2 (N10), 180 kg·hm-2 (N12), and 210 kg·hm-2 (N14), with an additional nitrogen-free treatment (N0) to calculate nitrogen efficiency. Four types of fertilizers were applied: compound fertilizer + urea (F1), slow-release mixed fertilizer (F2), controlled-release fertilizer (F3), and wheat formula fertilizer (F4). The effects of different treatments on wheat population dynamics, nitrogen utilization, yield, and quality were analyzed.

【Result】

As nitrogen application levels increased, the yield and quality indicators of weak-gluten wheat improved. The one-time basal application of slow-release mixed fertilizer, controlled-release fertilizer, and wheat formula fertilizer significantly increased the SPAD value of the flag leaf after anthesis and the number of spikes at maturity. Compared with the compound fertilizer+urea treatment, the nitrogen accumulation in the above-ground parts under slow-release mixed fertilizer, controlled-release fertilizer, and wheat formula fertilizer was increased by 5.0%-12.8%, 0.8%-6.2%, and 9.9%-17.2%, respectively; the nitrogen utilization efficiency increased by 10.3%-26.9%, 4.3%-16.5%, and 22.1%-39.8%, respectively; during anthesis, the activities of nitrate reductase (NR) in the flag leaf increased by 2.6%-8.8%, –2.9%-1.5%, and 5.1%-12.8%; glutamine synthetase (GS) activity increased by 9.0%-23.8%, 2.0%-8.9%, and 11.8%-28.7%; and glutamate synthase (GOGAT) activity increased by 3.9%-18.4%, 0.8%-8.9%, and 7.7%-24.0%; yield increased by 5.0%-11.5%, 1.8%-7.6%, and 9.7%-18.4%, respectively. At nitrogen levels of 180 and 210 kg·hm-2, slow-release mixed fertilizer, controlled-release fertilizer, and wheat formula fertilizer all resulted in varying degrees of yield improvement compared with the compound fertilizer+urea treatment. At the same nitrogen levels, slow-release mixed fertilizer and controlled-release fertilizer significantly reduced grain protein content, wet gluten content, grain hardness, and solvent retention capacity compared with the compound fertilizer + urea treatment. They also reduced dough formation time and stability time, lowered water absorption rate, and the farinograph quality number. These treatments also enhanced the dough rheological properties and weakened the gluten strength.

【Conclusion】

For weak-gluten wheat cultivation following rice in the Jiang-Huai region, it was recommended to control the nitrogen level at 180-210 kg·hm-2. A one-time basal application of slow-release mixed fertilizer could ensure both high quality and high yield of weak-gluten wheat.

Issue
An integrated physiology and proteomics analysis reveals the response of wheat grain to low temperature stress during booting
Journal of Integrative Agriculture (JIA) 2025, 24(1): 114-131
Published: 20 January 2025
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Low temperature (LT) in spring has become one of the principal abiotic stresses that restrict the growth and development of wheat. Diverse analyses were performed to investigate the mechanism underlying the response of wheat grain development to LT stress during booting. These included morphological observation, measurements of starch synthase activity, and determination of amylose and amylopectin content of wheat grain after exposure to treatment with LT during booting. Additionally, proteomic analysis was performed using tandem mass tags (TMT). Results showed that the plumpness of wheat grains decreased after LT stress. Moreover, the activities of sucrose synthase (SuS, EC 2.4.1.13) and ADP-glucose pyrophosphorylase (AGPase, EC 2.7.7.27) exhibited a significant reduction, leading to a significant reduction in the contents of amylose and amylopectin. A total of 509 differentially expressed proteins (DEPs) were identified by proteomics analysis. The Gene Ontology (GO) enrichment analysis showed that the protein difference multiple in the nutritional repository activity was the largest among the molecular functions, and the up-regulated seed storage protein (SSP) played an active role in the response of grains to LT stress and subsequent damage. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that LT stress reduced the expression of DEPs such as sucrose phosphate synthase (SPS), glucose-1-phosphate adenylyltransferase (glgC), and β-fructofuranosidase (FFase) in sucrose and starch metabolic pathways, thus affecting the synthesis of grain starch. In addition, many heat shock proteins (HSPs) were found in the protein processing in endoplasmic reticulum pathways, which can resist some damage caused by LT stress. These findings provide a new theoretical foundation for elucidating the underlying mechanism governing wheat yield development after exposure to LT stress in spring.

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