Shunbei oil/gas field is of the ultra-deep carbonate type controlled by multi-stage strike-slip faulting within the craton. The significant differences on the oil/gas property and abundance are shown in different fault zones even different segments in a fault zone. An integration of the core and thin section observation, cathodoluminescence, in-situ trace and rare earth element (REE) analysis, systematic analysis of fluid inclusions, is applied to study the sequence of fracture vein formation, sources of vein-forming fluids and their coupling relationships with hydrocarbon charging in the Middle-Lower Ordovician reservoir of the No. 1 and 5 strike-slip fault zones in Shunbei area. We also investigate the factors controlling differential hydrocarbon accumulation in Shunbei area while understanding the evolution of strike-slip faults and the hydrocarbon accumulation pattern. The results show that there are at least four phases of calcite vein development, that is, Cal-1, Cal-2, Cal-3, and Cal-4, respectively, in the Middle-Lower Ordovician reservoir of the No. 1 and 5 strike-slip fault zones in Shunbei area. The vein-forming fluids of Cal-1 are mainly sea-sourced fluids, while that of Cal-2, Cal-3 and Cal-4 are derived from diagenetic fluids in the reservoir where the veins occur. The formation of Cal-2, Cal-3 and Cal-4 calcite veins is associated with tectonic activities and hydrocarbon charging in the Late Caledonian to Early Hercynian, the Late Hercynian to Indosinian, and the Yanshan-Himalayan, respectively. The strike-slip fault zones are different in hydrocarbon charging, as shown by that the main charging phase of the No. 5 fault zone was during the Late Hercynian to Indosinian, while the main charging period of No. 1 fault zone was during the Late Hercynian to Indosinian, and Yanshan-Himalayan. The differential hydrocarbon charging controlled by strike-slip fault structural style and evolution history is the key to differential hydrocarbon accumulation in Shunbei area.