Hydrogen peroxide (H₂O₂) is widely used for disinfection because of its oxidative and antimicrobial properties. However, most studies have focused on surface disinfection, with limited research addressing its effectiveness for air disinfection. In this study, we developed a controlled air duct testing system to evaluate the disinfection performance of H₂O₂ in a ventilation system while precisely regulating environmental parameters. A sensitivity constant (Z) was introduced to quantitatively assess disinfection efficiency, with increased Z-values indicating enhanced disinfection performance. With a 7.5 s flow in the air duct system, the bioaerosol removal efficiency ranged from 26% to 97% under different temperature and humidity conditions. As temperature increased from 15 to 33 ℃, removal efficiency of Serratia marcescens and Escherichia coli increased from 40% to 95% and 78% to 97%, respectively. Correspondingly, Z-values increased from 0.0021 to 0.0076 ppm⁻¹s⁻¹ for S. marcescens and from 0.0072 to 0.0083 ppm⁻¹s⁻¹ for E. coli, suggesting that elevated temperature enhanced H₂O₂’s air disinfection efficiency. Conversely, higher humidities reduced H₂O₂’s efficacy; as the relative humidity decreased from 80% to 30%, the relative concentration of E. coli decreased and the Z-value dropped from 0.017 to 0.0022 ppm⁻¹s⁻¹. When the H₂O₂ concentration increased, the removal efficiency increased slightly, while the Z-value decreased significantly, indicating diminishing returns at higher H₂O₂ concentrations. These findings provide important insights for optimizing H₂O₂-based air disinfection strategies.

Bioaerosol filtration is crucial for improving indoor air quality and reducing cross-infection risk. It is necessary to conduct a comprehensive study on the bioaerosol filtration performance of air filters under different influencing factors. Firstly, this study proposed a standardized test scheme for the comprehensive evaluation of bioaerosol filtration performance. Additionally, the high-efficiency particulate air (H13) and medium-efficiency particulate air (F8) filters were evaluated in depth using Serratia marcescens bioaerosol, considering the face velocity, relative humidity (RH), and operating time. This study also investigated the impact of removing static electricity on the bioaerosol filtration efficiency (BFE) of F-filter materials. The pressure drop (∆P) value of H13-filter and the BFE of F8-filter were significantly affected by face velocity and RH. When the face velocity increased from 5 to 20 cm/s, the H13-filter maintained a BFE above 99%, while ∆P increased by 303 Pa. During a 90 min test, the maximum change in the BFE of the H13-filter was 0.80% and ∆P increased by 26 Pa. Conversely, the BFE of the F8-filter decreased by up to 12.21%, while ∆P increased by only 3 Pa. Higher RH resulted in more pronounced changes in BFE. After removing the static electricity from the F8-filter material, the BFE decreased significantly, with a maximum reduction of 25%. The results may provide valuable insights into the application of conventional filters in bioaerosol filtration and serve as a guide for the enhancement and optimization of filter design.