In order to improve the level of laboratory safety management and effectively control the occurrence of unsafe behaviors in university, this paper uses targeted intervention to control unsafe behaviors, including three steps: data analysis, node positioning, and policy implementation. Firstly, data information about time, individuals, types of laboratory, and unsafe behaviors are extracted from 131 laboratory accidents collected. Then, association rule is used to analyze the potential connections between each dimension, and the strong association rules are obtained. Finally, 10 specific intervention measures for the location node are proposed to provide reference for laboratory safety management. The research shows that laboratory accidents mostly occur in chemical laboratories, mainly due to experimental personnel's operational errors and incorrect wearing of safety protective equipment, in addition, accidents often occur at time nodes where students' activity patterns change greatly.