Microalgal-indigenous bacterial wastewater treatment (MBWT) emerges as a promising approach for the concurrent removal of nitrogen (N) and phosphorus (P). Despite its potential, the prevalent use of MBWT in batch systems limits its broader application. Furthermore, the success of MBWT critically depends on the stable self-adaptation and synergistic interactions between microalgae and indigenous bacteria, yet the underlying biological mechanisms are not fully understood. Here we explore the viability and microbial dynamics of a continuous flow microalgae-indigenous bacteria advanced wastewater treatment system (CFMBAWTS) in processing actual secondary effluent, with a focus on varying hydraulic retention times (HRTs). The research highlights a stable, mutually beneficial relationship between indigenous bacteria and microalgae. Microalgae and indigenous bacteria can create an optimal environment for each other by providing essential cofactors (like iron, vitamins, and indole-3-acetic acid), oxygen, dissolved organic matter, and tryptophan. This collaboration leads to effective microbial growth, enhanced N and P removal, and energy generation. The study also uncovers crucial metabolic pathways, functional genes, and patterns of microbial succession. Significantly, the effluent NH₄⁺-N and P levels complied with the Chinese national Class-II, Class-V, Class-IA, and Class-IB wastewater discharge standards when the HRT was reduced from 15 to 6 h. Optimal results, including the highest rates of CO₂ fixation (1.23 g L⁻¹), total energy yield (32.35 kJ L⁻¹), and the maximal lipid (33.91%) and carbohydrate (41.91%) content, were observed at an HRT of 15 h. Overall, this study not only confirms the feasibility of CFMBAWTS but also lays a crucial foundation for enhancing our understanding of this technology and propelling its practical application in wastewater treatment plants.

Conventional biological treatment usually cannot achieve the same high water quality as advanced treatment when conducted under varied temperatures. Here, satisfactory wastewater treatment efficiency was observed in a microalgae-bacteria consortia (MBC) over a wide temperature range because of the predominance of microalgae. Microalgae contributed more toward wastewater treatment at low temperature because of the unsatisfactory performance of the accompanying bacteria, which experienced cold stress (e.g., bacterial abundance below 3000 sequences) and executed defensive strategies (e.g., enrichment of cold-shock proteins). A low abundance of amoA-C and hao indicated that conventional nitrogen removal was replaced through the involvement of microalgae. Diverse heterotrophic bacteria for nitrogen removal were identified at medium and high temperatures, implying this microbial niche treatment contained diverse flexible consortia with temperature variation. Additionally, pathogenic bacteria were eliminated through microalgal photosynthesis. After fitting the neutral community model and calculating the ecological niche, microalgae achieved a maximum niche breadth of 5.21 and the lowest niche overlap of 0.38, while the accompanying bacterial community in the consortia were shaped through deterministic processes. Finally, the maximum energy yield of 87.4 kJ L⁻¹ and lipid production of 1.9 g L⁻¹ were achieved at medium temperature. Altogether, this study demonstrates that advanced treatment and energy reclamation can be achieved through microalgae-bacteria niche strategies.

Efficient wastewater treatment accompanied by sustainable “nutrients/pollutants waste-wastewater-resources/energy nexus” management is acting as a prominent and urgent global issue since severe pollution has occurred increasingly. Diverting wastes from wastewater into the value-added microalgal-biomass stream is a promising goal using biological wastewater treatment technologies. This review proposed an idea of upgrading the current microalgal wastewater treatment by using immobilized microalgal system. Firstly, a systematic analysis of microalgal immobilization technology is displayed through an in-depth discussion on why using immobilized microalgae for wastewater treatment. Subsequently, the main technical approaches employed for microalgal immobilization and pollutant removal mechanisms by immobilized microalgae are summarized. Furthermore, from high-tech technologies to promote large-scale production and application potentials in diverse wastewater and bioreactors to downstream applications lead upgradation closer, the feasibility of upgrading existing microalgal wastewater treatment into immobilized microalgal systems is thoroughly discussed. Eventually, several research directions are proposed toward the future immobilized microalgal system for microalgal wastewater treatment upgrading. Together, it appears that using immobilization for further upgrading the microalgae-based wastewater treatment can be recognized as an achievable alternative to make microalgal wastewater treatment more realistic. The information and perspectives provided in this review also offer a feasible reference for upgrading conventional microalgae-based wastewater treatment.