Pt single atoms catalysts with precise coordination environment and high stability are expected to achieve high performance of propane dehydrogenation (PDH). In this work, an innovative synthetic strategy is proposed to construct the S-1@0.1Pt9Zn@DPS-1 nanocomposite as a highly efficient PDH catalyst. Defect engineering is applied to induce the formation of defective porous silicalite-1 (DPS-1), which is favorable for achieving the uniformly distributed ZnO nanoclusters. The ZnO nanoclusters were further served as anchoring sites to stabilize the isolated Pt atoms. The structural characterization revealed that penta-O coordinated Pt single atom coupled with ZnO nanoclusters decorated on the DPS-1. Moreover, the atomically dispersed Pt atoms with the ideal coordination environment could act as the predominant active sites for PDH process. As expected, the optimal S-1@0.1Pt9Zn@DPS-1 catalyst delivered an excellent PDH performance (propane conversion of 40.7%, propylene selectivity of 97.5%) and good cycling regeneration stability. This work provides a new way for improving the activity and stability of catalysts in the field of industrial catalysts.