This paper sought to address the prevalence of Mucorales in different indoor environments in Portugal. Environmental samples (183 in total) were collected at dwellings (n = 79) and workplaces (bakeries, swine farms, taxis, waste-sorting plants) (n = 93) by passive sampling using electrostatic dust collector (EDC), air-conditioning filters, litter, and/or raw materials. Samples were inoculated onto non-selective MEA and DG18 media and were screened for antifungal drug-resistance in azole-supplemented agar Sabouraud media. A probe-based Mucorales-specific real-time PCR assay (Muc18S) was used to detect Mucorales in complement to conventional culture-based methods. Mucorales order was found as more prevalent in air-conditioning filters from waste-sorting fork lifters (35.7%). Amongst Mucorales isolates able to grow in azole-supplemented media, 16 isolates of Mucor sp., Rhizopus sp. or Rhizomucor sp. were not susceptible to 1 mg/L voriconazole, and four isolates of Mucor sp. or Rhizopus sp. were not susceptible to 4 mg/L itraconazole. In conclusion, combination of the culture-based and molecular methods proved to be reliable for Mucorales order identification in complex environmental samples.

Sterigmatocystin (STC) is a possible human carcinogen (2B) according to International Agency for Research on Cancer classification and has been associated with immunotoxic and immunomodulatory activity, together with mutagenic effects. It might be found in numerous substrates, from foods and feeds to chronically damp building materials and indoor dust. Although European Food Safety Authority concluded that the exposure to STC to be of low concern for public health, reinforces the need of data concerning exposure of European citizens. Climate change can represent an increased risk of exposure to STC since it is a crucial factor for agro-ecosystem powering fungal colonisation and mycotoxin production This aspect can represent an increased risk for European countries with temperate climates and it was already reported by the scientific community.