Investigation on bioavailability was carried out by determining the absorption and transport of bioaccessible apigenin from fruit of mango (Mangifera indica var. Water Lily) into Caco-2 human intestinal cell using a reliable and sensitive analytical method of LC-MS/MS. Results revealed that the concentration of glucuronidated apigenin was lower than apigenin. The apigenin was metabolised inside the cells through glucuronidation process, and cross the monolayer to reach the basolateral sides or effluxed back to the apical side. The permeability coefficient of apigenin from apical to basolateral sides and basolateral to apical sides showed a medium permeability of less than 20 ×10⁻⁶ cm·sec⁻¹. Since the value of efflux ratio was 1.5, it suggested that apigenin was absorbed and transported through a simple diffusion mechanism.