Degrading purine nucleosides in wort into free purines to facilitate yeast absorption has been proven effective for reducing purine in beer. In this study, a probiotic strain of Pediococcus acidilactici GR-5, isolated from traditional fermented foods in northwest China, was found to completely degrade adenosine, guanosine, and inosine within 12 h. Using GR-5 post-fermentation to produce beer, the contents of purine nucleoside and purine in beer were reduced by approximately 80.15% and 41.91%, respectively. The purine metabolic pathway of GR-5 was upregulated during the fermentation process, enhancing the beer’s fruity flavor with minimal impact on overall volatile flavor. GR-5 in low-purine live bacteria beer mitigates beer-induced serum UA elevation, liver and kidney damage, and inflammatory factor levels by regulating renal UA synthase, antioxidant indicators, and the NLRP3 inflammatory pathways. GR-5 also colonizes the mouse intestine, enhancing the purine metabolism of gut microbiota and increasing the abundance of probiotics. This technology offers a novel approach for the production and technical optimization of low-purine beer.
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Phenanthrene is one of the most recalcitrant components of crude oil-contaminated wastewater. An efficient phenanthrene-degrading bacteriumPseudomonas sp. strain named LZ-Q was isolated from oil-contaminated soil near the sewage outlet of a petrochemical company.Pseudomonas sp. LZ-Q is able to degrade 1000 mg/L phenanthrene in Bushnell-Hass mineral salt medium. It also degrades other polycyclic aromatic hydrocarbons such as naphthalene, anthracene, pyrene, petrol, and diesel at broad ranges of salinities of 5 g/L to 75 g/L, pHs of 5.0–10.0, and temperatures of 10–42 °C. Therefore,Pseudomonas sp. LZ-Q could be a good candidate for remediation of polycyclic aromatic hydrocarbon (PAH)-contaminated wastewater. A membrane bioreactor (MBR) was applied to investigate the remediation ability of the strain LZ-Q. Wastewater containing phenanthrene with pH of 8, salinity of 35 g/L, and COD of 500 mg/L was continuously added to the system (HRT = 3 h). Results showed thatPseudomonas sp. LZ-Q is capable of degrading 96% of 20 mg/L phenanthrene and 94% of 500 mg/L COD for 60 days in a continuous mode. These results showed that the MBR system with strain LZ-Q might be a good approach for PAHs’ remediation in industrial wastewaters.
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