Insulin resistance, a prominent characteristic of type 2 diabetes, has been extensively studied. In our investigation, we examined the effect of Eudesmin on insulin resistance in 3T3-L1 adipocytes and HepG2 cells. Additionally, we analyzed the alterations in mRNA expression in insulin-resistant HepG2 cells through transcriptomic techniques. Subsequently, we assessed the changes in mRNA expression in insulin-resistant HepG2 cells following Eudesmin treatment. Our analysis revealed 13 differentially expressed genes that were commonly observed. Notably, FER1L6 exhibited the most significant changes, with a marked upregulation in mRNA expression in insulin-resistant HepG2 cells. Consequently, we conducted functional experiments to validate the role of fer-1 like family member 6 (FER1L6). Specifically, through gene knockdown experiments, we observed a significant enhancement in glucose transporter type 4 (GLUT4) expression and an improvement in insulin resistance status in cell models. Therefore, FER1L6 emerges as a promising therapeutic target for insulin resistance.

Human health is threatened by foodborne illness and Staphylococcus aureus is a common foodborne pathogenic bacteria. It can cause food poisoning when we are infected, therefore, it is necessary to detect pathogenic bacteria. The virulence genes and detection methods of S. aureus are summarized from literatures. Traditional detection methods are simple but need bacteria enrichment and to prolong detection time. Immunological technology has high specificity, but false positive results are easy to occur in its detection. In recent years, molecular biology methods have developed rapidly, and various PCR techniques have been applied to detect foodborne pathogens. Real-time fluorescent quantitative PCR is more effective than ordinary PCR, while price is expensive when use it, the scope of use is limited. LAMP is a flexible detection method, biosensor and flow cytometry are fast but the cost of detection is high and the price is relatively expensive.