Organic light-emitting transistors (OLETs) integrate the functions of light-emitting diodes and field-effect transistors into a unique device, opening a new door for optoelectronics. However, there is still a challenge due to the absence of high quality organic semiconductors for OLETs. Herein, we reported a novel molecule 2,6-di(anthracen-2-yl)naphthalene (2,6-DAN), which exhibited mobility of up to 19 cm²·V^-1·s^-1 and an absolute fluorescence quantum yield of 37.09%, which are good values for organic semiconductors. Moreover, OLETs based on 2,6-DAN single crystals showed bright yellowish-green emission and well-balanced ambipolar charge transport. The excellent ratio of hole to electron mobility can reach up to 0.86, which is superior to most single-component OLETs in typical device configurations reported so far.