We demonstrate that a recently developed coating composed of superhydrophobic silicone nanofilaments can be selectively functionalized to yield well defined micron-scale patterns of contrasting wettabilities (superhydrophobic/hydrophilic and amphiphobic/amphiphilic). Nanofilament ablation was performed using a near-ultraviolet (UV) laser with a wavelength of 355 nm and a repetition rate of 10 kHz. This is a highly promising approach for open channel microfluidics and microarray analysis due to its simplicity, the chemical and environmental stability of the coating, and the low cost.