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Open Access Original Research Issue
Research on the distal repair effect of Jiawei Buyang Huanwu Decoction on sciatic nerve injury through agrin signaling pathway
Journal of Neurorestoratology 2023, 11 (2): 100058
Published: 14 May 2023
Downloads:23
Objective

To study the effects and potential control mechanisms of the agrin signaling pathway on the repair of distal sciatic nerve damage.

Methods

50 Sprague-Dawleyrats were randomly allocated into the control group (SCI group, n = 25) and the treatment group (Treat group, n = 25). In both groups, the sciatic nerve damage model was created. Following surgery, the SCI group and the Treat group received intragastric administrations of normal saline and Jiawei Buyang Huanwu Decoction, respectively. The dose for both groups was 2mL/(kg·d) for 12 consecutive weeks. At 4, 8, and 12 weeks post-surgery, the wet-to-weight ratio of the gastrocnemius muscle in the two groups was assessed; and hematoxylin-eosin staining and Masson staining were used to detect pathological alterations in the sciatic nerve and collagen. The expression of agrin, Dag1, Dmd, and Lamb2 proteins in the agrin pathway, as well as ERK, MEK, and their corresponding phosphorylated proteins, p-ERK and p-MEK, were all detected by Western blot in the gastrocnemius muscle.

Results

The wet weight of the gastrocnemius muscle in the Treat group increased considerably (p < 0.05) after 4, 8, and 12 weeks following the operation compared to the SCI group, and it continued to rise over time. The expression level of Dag1, Dmd, and Lamb2 protein in agrin pathway, as well as p-ERK and p-MEK protein were all significantly lower than they were in the SCI group (p < 0.05). Additionally, Jiawei Buyang Huanwu Decoction greatly reduced the amount of nerve scars at the sciatic nerve injury and significantly healed sciatic neuropathy according to the results of pathological staining.

Conclusion

Jiawei Buyang Huanwu Decoction can inhibit the expression of agrin, Dag1, Dmd, and Lamb2, as well as p-ERK and p-MEK. It can effectively promote the distal repair of sciatic nerve injury probably through argin pathway.

Open Access Research Article Issue
Clinical observation of effects of ultrashort wave therapy combined with acupuncture and rehabilitation training in the treatment of patients with dysphagia after stroke
Journal of Neurorestoratology 2019, 7 (3): 136-142
Published: 05 September 2019
Downloads:26
Aim:

The present study aimed to assess the clinical effects of ultrashort wave therapy combined with acupuncture and rehabilitation training on patients with dysphagia after stroke.

Method:

A total of 126 patients with stroke with dysphagia were randomly divided into an acupuncture group (control group: 63 patients) and a comprehensive rehabilitation training group (treatment group: 63 patients). The control group received rehabilitation training and acupuncture, whereas the treatment group received ultrashort wave therapy in addition to rehabilitation training and acupuncture (comprehensive rehabilitation training). The curative effect was evaluated using water-drinking test scores and swallowing quality of life scale (SWAL-QOL) scores before and after intervention. Additionally, the incidence of aspiration pneumonia was assessed in the two groups.

Results:

The water-drinking test scores in both groups were significantly better after 4 weeks of intervention than before intervention (P < 0.01); however, the improvement degree was significantly greater in the treatment group than in the control group (P < 0.01). The SWAL-QOL scores in both groups were significantly higher after intervention than before intervention (P < 0.05); however, the improvement degree was significantly greater in the treatment group than in the control group (P < 0.05). Moreover, the incidence of aspiration pneumonia was significantly lower in the treatment group than in the control group (P < 0.05).

Conclusion:

Comprehensive rehabilitation training can greatly improve dysphagia after stroke and can effectively reduce the incidence of aspiration pneumonia.

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