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Publishing Language: Chinese | Open Access

Rapid Visual Detection of Trace Pseudomonas aeruginosa in Packaged Drinking Water Using Nucleic Acid Test Strips

Xianzhuo MENG1 , Chao YAN2, Jing ZHANG3, Bangben YAO3 ( ), Zhaoran CHEN3, Qingli YANG1, Wei CHEN1 ( )
School of Food and Bioengineering, Hefei University of Technology, Hefei 230601, China
School of Life Sciences, Anhui University, Hefei 230601, China
Anhui Institute of Product Quality Supervision and Inspection, Hefei 230051, China
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Abstract

Objective

To establish a new method for the rapid detection of Pseudomonas aeruginosa in packaged drinking water using colloidal gold-based lateral flow immunochromatographic strips.

 Methods

1 mg/mL sheep anti-mouse antibody and 1 mg/mL streptavidin (SAV) were sprayed onto a nitrocellulose (NC) membrane by using a membrane sprayer at a rate of 0.5 μL/cm, and then the antibody labeled with fluorescein isothiocyanate (FITC) was conjugated to the surface of colloidal gold by electrostatic adsorption, and the resulting conjugate was added dropwise to the gold standard pad. The amplification products were added dropwise to the fully assembled lateral flow immunochromatographic strip, and the results were judged based on the positions of the control (C) and test (T) lines generated.

 Results

The method was able to specifically identify P. aeruginosa in packaged drinking water in 3 min with a detection limit of 1 CFU/250 mL, which was more sensitive than agarose gel electrophoresis.

 Conclusion

The lateral flow immunoassay method was characterized by high sensitivity, high specificity, simplicity and portability, and was suitable for real samples.

Keywords

Pseudomonas aeruginosapackaging drinking waterlateral flow immunochromatographic striprapid detection
CLC number: TS207.3 Document code: A Article ID: 1002-6630(2024)15-0229-08

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Food Science
Volume 45 Issue 15,
August 2024
Pages 229-236
DOI: 10.7506/spkx1002-6630-20231215-124

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Cite this article:
MENG X, YAN C, ZHANG J, et al. Rapid Visual Detection of Trace Pseudomonas aeruginosa in Packaged Drinking Water Using Nucleic Acid Test Strips. Food Science, 2024, 45(15): 229-236. https://doi.org/10.7506/spkx1002-6630-20231215-124

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Received: 15 December 2023
Published: 15 August 2024
© Beijing Academy of Food Sciences 2024.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).