Whole Genome Analysis and Functional Prediction of Ligilactobacillus cholophilus BD7642

A novel strain of Ligilactobacillus cholophilus, designated BD7642, was isolated from pickled potherb mustard (Brassica juncea Coss.) in Shanghai. The complete genome sequence of the strain was obtained using a combination of PacBio and Illumina sequencing. To characterize this strain and explore its potential probiotic functions, we performed comprehensive genomic analyses. The results showed that the genome of L. cholophilus BD7642 was 1587935 bp in size and encoded 1530 genes (no plasmids detected). Functional annotation and secondary metabolite synthesis analysis revealed that L. cholophilus BD7642 contained gene clusters for the biosynthesis of the bacteriocin mutacin 1140 and type Ⅲ polyketide synthases and possessed a great capacity for environmental adaptation. ResFinder analysis indicated the absence of antibiotic resistance genes. Comparative genomics uncovered the genomic uniqueness of L. cholophilus BD7642. This study provides a deep understanding of L. cholophilus BD7642 and offers a reference for exploiting its probiotic potential.