Identification of Peptoniphilus asaccharolyticus Isolated from Spoiled Fermented Milk Intended for Students

This study focuses on the identification of Peptoniphilus asaccharolyticus from spoiled fermented milk intended for students based on isolation, counting, morphological observation, biochemical identification, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), and whole-genome sequencing. The results showed that the isolate grew well on PCA medium under anaerobic conditions and was identified as P. asaccharolyticus on a fully automated microbial identification system with a confidence level of 98%. By MALDI-TOF MS, it was confirmed as P. sasaccharolyticus with a comparison value of 2.241. The result of whole genome sequencing showed an average nucleotide identity (ANI) of 98.58%. The proposed method allows rapid and accurate identification of P. sasaccharolyticus and can meet the requirements of food safety detection.