Uncertainty Evaluation for the Determination of Nicotinamide in Milk Powder by Ultra-High Performance Liquid Chromatography

The uncertainty in the determination of nicotinamide in milk powder by ultra-high performance liquid chromatography was evaluated. According to the technical normal “Evaluation and Expression of Uncertainty in Measurement” (JJF 1059.1-2012), a mathematical model was established, and the source of each uncertainty component in the mathematical model was analyzed and calculated. The results showed that the uncertainty in nicotinamide determination mainly came from the dilution of the standard solution. In addition, the average content of nicotinamide in milk powder was 5007 μg/100 g with an expanded uncertainty of about 197 μg/100 g (k = 2).