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Basic Research | Publishing Language: Chinese | Open Access

Effects of Tea Polyphenols on Conformation and Antigenicity of Bovine Milk αs1-Casein Allergen

Honglei GUO1 , Shengyun ZHOU1, Yanjun CONG1 ( ), Wenjie YAN2
Beijing Research Center for Food Additive Engineering Technology, Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food and Health, Beijing Technology and Business University, Beijing 100048, China
College of Biochemical Engineering, Beijing Union University, Beijing 100023, China
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Abstract

In order to investigate the effects of tea polyphenols on the conformation and antigenicity of αs1-casein, epigallocatechin gallate (EGCG) and epigallocatechin (EGC), tea polyphenols with high bioactivity, were used for grafting onto αs1-casein. The conformational changes of αs1-casein were studied by fluorescence, synchronous fluorescence and circular dichroism (CD) spectroscopy, and the changes in the antigenicity of αs1-casein were analyzed by indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and Western blot assay. The results showed that EGC and EGCG both quenched the fluorescence of αs1-casein, and affected the Tyr and Trp residues of αs1-casein. The α-helix and β-sheet contents of αs1-casein increased slightly, and the random coil content decreased slightly. The conformational change of αs1-casein resulted in a significant decrease in the antigenicity of αs1-casein. The effect of EGCG on the conformation and antigenicity of αs1-casein was stronger than that of EGC.

Keywords

αs1-caseinepigallocatechin gallateepigallocatechinconformationantigenicity
CLC number: TS252.1 Document code: A Article ID: 1671-5187(2022)04-0007-07

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Journal of Dairy Science and Technology
Volume 45 Issue 4,
July 2022
Pages 7-13
DOI: 10.7506/rykxyjs1671-5187-20220508-026

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GUO H, ZHOU S, CONG Y, et al. Effects of Tea Polyphenols on Conformation and Antigenicity of Bovine Milk αs1-Casein Allergen. Journal of Dairy Science and Technology, 2022, 45(4): 7-13. https://doi.org/10.7506/rykxyjs1671-5187-20220508-026

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Received: 08 May 2022
Published: 01 July 2022
© Bright Dairy & Food Co., Ltd. 2022.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).