The Anti-proliferative Activity of Factory Wastes Nanoparticles against Uterus Cancer Cells: In-vitro Study

As a result of increasingly rigorous environmental legislation and industry adoption of waste minimization recommendations, the development of recycling and reuse possibilities for metal-related trash has become increasingly important.Inthis research, calcium oxide and mixture of calcium, iron, and magnesium oxides was extracted from the wastes of cement factories using hydrofluoric acid. As a result, this research provides a comprehensive picture of waste recycling, and reuse throughout extraction and it examines the current state and potential developments in mineral and metal waste recycling and reusing them in different applications, among these applications, it is used CaO and CaO:MgO:Fe₂O₃ NPs in medical applications, including uterine cancer. Characterizations of the extracted nano-products were done using (XRD) which confirmed the crystalline nature of CaO and the mixture of CaO:MgO:Fe₂O₃ NPs. More, (FESEM) has been used to demonstrate the morphology of the nanostructures of CaO and the mixture CaO:MgO:Fe₂O₃ NPs. The data yielded the average grain size of CaO and the mixture CaO:MgO:Fe₂O₃ NPs calculated by SEM and XRD (64.08, 84.2) nm and (38.636, 31.597) nm respectively. (EDS) spectrum and XRD pattern suggested that prepared CaO and the mixed CaO:MgO:Fe₂O₃ NPs were highly pure. In addition, the ability of NPs as a promising anticancer agent was tested against MES-SA cells line, according to the MTT assay results of MES-SA cells the I_C50 (91.65 and 98.4) μg/mL for CaO and CaO:MgO:Fe₂O₃ NPs respectively. The results showed that the extracted nanoparticles CaO and the mixture CaO:MgO:Fe₂O₃ NPs served as an anticancer agent.