AI Chat Paper
Note: Please note that the following content is generated by AMiner AI. SciOpen does not take any responsibility related to this content.
{{lang === 'zh_CN' ? '文章概述' : 'Summary'}}
{{lang === 'en_US' ? '中' : 'Eng'}}
Chat more with AI
PDF (8.7 MB)
Collect
Submit Manuscript AI Chat Paper
Show Outline
Outline
Show full outline
Hide outline
Outline
Show full outline
Hide outline
Research Article | Open Access

In vivo Antioxidant Potential of Biogenic Silver Nanoparticles Synthesized from Psidium guajava L.

Atif Yaqub1( )Muhammad Rashid1Sarwar Allah Ditta1Naila Malkani1Nazish Mazhar Ali1Muhammad Zubair Yousaf2Arslan Haider1Muhammad Jamil Yousaf3Saman Abdullah1
Department of Zoology, Government College University, Lahore 54000, Pakistan
School of Life Sciences, Forman Christian College University, Lahore 54600, Pakistan
Higher Education Department, Government of the Punjab, Lahore 54000, Pakistan
Show Author Information

Graphical Abstract

Abstract

Excessive utilization of nanoparticles renders it necessary to produce safer and more secure nanoparticles while preserving their efficacy. In this study, Psidium guajava L. pulp extract-mediated silver nanoparticles (AgNPs) were synthesized, characterized, and further evaluated regarding their antioxidant potential. The green synthesized silver nanoparticles (G-AgNPs) showed a higher level of radical scavenging activity (RSA) (25.85%), hydrogen peroxide scavenging activity (34.34%), and ferric reducing power (0.28). The comparison of the control group (G1) with the various treatment groups (G2–G6) revealed that the levels of catalase (CAT), superoxide dismutase (SOD), and glutathione-S-transferase (GST) were significantly different (P < 0.05). The levels of blood urea, uric acid, blood urea nitrogen (BUN), serum glutamic pyruvic transaminase (SGTP), serum creatinine, serum glutamic-oxaloacetic transaminase (SGOT), alkaline phosphatase, total bilirubin, and serum electrolytes were also evaluated. The results of clinical biochemistry also strengthened our hypothesis that G-AgNPs are less toxic than C-AgNPs. Finally, the histopathology of liver, kidney, and intestinal tissues indicated that green-synthesized AgNPs are relatively safer.

References

[1]
M. Saravanan, H. Barabadi, H. Vahidi. Green nanotechnology: isolation of bioactive molecules and modified approach of biosynthesis. In: Biogenic Nanoparticles for Cancer Theranostics. Amsterdam: Elsevier, 2021: 101–122.
[2]
O.N. Kanwugu, M.N. Ivantsova, K.D. Chidumaga. Gold biotechnology: development and advancements. In: AIP Conference Proceedings. 2018.
[3]

U. Chadha, P. Bhardwaj, R. Agarwal, et al. Recent progress and growth in biosensors technology: A critical review. Journal of Industrial and Engineering Chemistry, 2022, 109: 21−51. https://doi.org/10.1016/j.jiec.2022.02.010

[4]

H. Barabadi, H. Noqani, F. Ashouri, et al. Nanobiotechnological approaches in anticoagulant therapy: The role of bioengineered silver and gold nanomaterials. Talanta, 2023, 256: 124279. https://doi.org/10.1016/j.talanta.2023.124279

[5]

S. Majeed, M. Saravanan, M. Danish, et al. Bioengineering of green-synthesized TAT peptide-functionalized silver nanoparticles for apoptotic cell-death mediated therapy of breast adenocarcinoma. Talanta, 2023, 253: 124026. https://doi.org/10.1016/j.talanta.2022.124026

[6]

N. Talank, H. Morad, H. Barabadi, et al. Bioengineering of green-synthesized silver nanoparticles: In vitro physicochemical, antibacterial, biofilm inhibitory, anticoagulant, and antioxidant performance. Talanta, 2022, 243: 123374. https://doi.org/10.1016/j.talanta.2022.123374

[7]

H. Barabadi, A. Mohammadzadeh, H. Vahidi, et al. Penicillium chrysogenum-derived silver nanoparticles: Exploration of their antibacterial and biofilm inhibitory activity against the standard and pathogenic acinetobacter baumannii compared to tetracycline. Journal of Cluster Science, 2022, 33(5): 1929−1942. https://doi.org/10.1007/s10876-021-02121-5

[8]

S. Sarina, E.R. Waclawik, H. Zhu. Photocatalysis on supported gold and silver nanoparticles under ultraviolet and visible light irradiation. Green chemistry, 2013, 15(7): 1814−1833. https://doi.org/10.1039/C3GC40450A

[9]

A.C. Burdușel, O. Gherasim, A.M. Grumezescu, et al. Biomedical applications of silver nanoparticles: An up-to-date overview. Nanomaterials, 2018, 8(9): 681. https://doi.org/10.3390/nano8090681

[10]

M. Ovais, A.T. Khalil, N.U. Islam, et al. Role of plant phytochemicals and microbial enzymes in biosynthesis of metallic nanoparticles. Applied Microbiology and Biotechnology, 2018, 102(16): 6799−6814. https://doi.org/10.1007/s00253-018-9146-7

[11]
G.A. Engwa. Free radicals and the role of plant phytochemicals as antioxidants against oxidative stress-related diseases. In: Phytochemicals: Source of Antioxidants and Role in Disease Prevention. InTech, 2018.
[12]

J.N. Moloney, T.G. Cotter. ROS signalling in the biology of cancer. Seminars in Cell &Developmental Biology, 2018, 80: 50−64. https://doi.org/10.1016/j.semcdb.2017.05.023

[13]

M. Dumont, M.F. Beal. Neuroprotective strategies involving ROS in Alzheimer disease. Free Radical Biology &Medicine, 2011, 51(5): 1014−1026. https://doi.org/10.1016/j.freeradbiomed.2010.11.026

[14]

C.T. Hong, C.J. Hu, H.Y. Lin, et al. Effects of concomitant use of hydrogen water and photobiomodulation on Parkinson disease. Medicine, 2021, 100(4): e24191. https://doi.org/10.1097%2FMD.0000000000024191

[15]

A.J. Kattoor, N.V.K. Pothineni, D. Palagiri, et al. Oxidative stress in atherosclerosis. Current Atherosclerosis Reports, 2017, 19(11): 42. https://doi.org/10.1007/s11883-017-0678-6

[16]

J. Zou, Q. Fei, H. Xiao, et al. VEGF-A promotes angiogenesis after acute myocardial infarction through increasing ROS production and enhancing ER stress-mediated autophagy. Journal of Cellular Physiology, 2019, 234(10): 17690−17703. https://doi.org/10.1002/jcp.28395

[17]

E. Nur, B.J. Biemond, H.M. Otten, et al. Oxidative stress in sickle cell disease; pathophysiology and potential implications for disease management. American Journal of Hematology, 2011, 86(6): 484−489. https://doi.org/10.1002/ajh.22012

[18]

İ. Gulcin. Antioxidants and antioxidant methods: An updated overview. Archives of Toxicology, 2020, 94(3): 651−715. https://doi.org/10.1007/s00204-020-02689-3

[19]

Z. Bedlovičová, I. Strapáč, M. Baláž, et al. A brief overview on antioxidant activity determination of silver nanoparticles. Molecules, 2020, 25(14): 3191. https://doi.org/10.3390/molecules25143191

[20]

N.S.R. Rosman, N.A. Harun, I. Idris, et al. Eco-friendly silver nanoparticles (AgNPs) fabricated by green synthesis using the crude extract of marine polychaete, Marphysa moribidii: biosynthesis, characterisation, and antibacterial applications. Heliyon, 2020, 6(11): e05462. https://doi.org/10.1016/j.heliyon.2020.e05462

[21]
L. Manzocco, M. Anese, M. Nicoli. Antioxidant properties of tea extracts as affected by processing. LWT-Food Science and Technology, 1998, 31(7–8): 694–698. https://doi.org/10.1006/fstl.1998.0491
[22]

R.J. Ruch, S.J. Cheng, J.E. Klaunig. Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis, 1989, 10(6): 1003−1008. https://doi.org/10.1093/carcin/10.6.1003

[23]
G.K. Jayaprakasha, R.P. Singh, K.K. Sakariah. Antioxidant activity of grape seed (Vitis vinifera) extracts on peroxidation models in vitro. Food Chemistry, 2001, 73(3): 285–290.
[24]
OECD. Test No. 407: Repeated Dose 28-day Oral Toxicity Study in Rodents, OECD Guidelines for the Testing of Chemicals, Section 4. OECD Publishing: Paris, 2018.
[25]

S. Marklund, G. Marklund. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. European Journal of Biochemistry, 1974, 47(3): 469−474. https://doi.org/10.1111/j.1432-1033.1974.tb03714.x

[26]
R.A. Greenwald. CRC handbook of methods for oxygen radical research. Boca Raton, Fla.: CRC Press, 1985.
[27]

M. Javed, I. Ahmad, N. Usmani, et al. Studies on biomarkers of oxidative stress and associated genotoxicity and histopathology in Channa punctatus from heavy metal polluted canal. Chemosphere, 2016, 151: 210−219. https://doi.org/10.1016/j.chemosphere.2016.02.080

[28]

Z. Guan, S. Ying, P.C. Ofoegbu, et al. Green synthesis of nanoparticles: Current developments and limitations. Environmental Technology &Innovation, 2022, 26: 102336. https://doi.org/10.1016/j.eti.2022.102336

[29]

S. Karimarji, S.G. Sabouri, A. Khorsandi. Nonlinear optical properties of size-variable silver nanoparticles dispersed in water and potassium bromide. Optical and Quantum Electronics, 2022, 54(2): 1−16. https://doi.org/10.1007/s11082-022-03526-w

[30]

M. Vanaja, G. Annadurai. Coleus aromaticus leaf extract mediated synthesis of silver nanoparticles and its bactericidal activity. Applied nanoscience, 2013, 3(3): 217−223. https://doi.org/10.1007/s13204-012-0121-9

[31]
D. Raju, N. Paneliya, U.J. Mehta, Extracellular synthesis of silver nanoparticles using living peanut seedling. Applied Nanoscience, 2014, 4(7): 875–879.
[32]

D. Bose, S. Chatterjee. Antibacterial activity of green synthesized silver nanoparticles using vasaka (Justicia adhatoda L.) leaf extract. Indian Journal of Microbiology, 2015, 55(2): 163−167. https://doi.org/10.1007/s12088-015-0512-1

[33]

V. Sandhiya, B. Gomathy, M.R. Sivasankaran, et al. Green synthesis of silver nanoparticles from Guava (Psidium guajava Linn.) leaf for antibacterial, antioxidant and cytotoxic activity on HT-29 cells (Colon cancer). Annals of the Romanian Society for Cell Biology, 2021, 25(6): 20148−20163.

[34]
C. Anggraini. Study antioxidant activity of silver nanoparticles prepared from guava leaves and fruits (Psidium guajava L.). 2017. https://www.semanticscholar.org/author/C.-Anggraini/1475182424 (accessed on June 15, 2023)
[35]
D.S.R. Tenkayala, P.R. Sougandhi, M. Reddeppa, et al. Green synthesis and characterization of silver nano particles by using psidium guajava leaf extract. Journal of Drug Delivery and Therapeutics, 2018, 8(5-s): 301–305.
[36]
N.A. Zamanhuri, R. Alrozi, M.S. Osman, et al. Green synthesis and characterizations of silver and gold nanoparticles using pink guava waste extract (PGWE). In: 2012 IEEE Symposium on Humanities, Science and Engineering Research. 2012: 45–48.
[37]

V.-T. Hoang, N.X. Dinh, N.L.N. Trang, et al. Functionalized silver nanoparticles-based efficient colorimetric platform: Effects of surface capping agents on the sensing response of thiram pesticide in environmental water samples. Materials Research Bulletin, 2021, 139: 111278. https://doi.org/10.1016/j.materresbull.2021.111278

[38]

B. Sadeghi, F. Gholamhoseinpoor. A study on the stability and green synthesis of silver nanoparticles using Ziziphora tenuior (Zt) extract at room temperature. Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy, 2015, 134: 310−315. https://doi.org/10.1016/j.saa.2014.06.046

[39]

H. Shumail, S. Khalid, I. Ahmad, et al. Review on green synthesis of silver nanoparticles through plants. Endocrine,Metabolic &Immune Disorders - Drug Targets, 2021, 21(6): 994−1007. https://doi.org/10.2174/1871530320666200729153714

[40]
V.V. Makarov, A.J. Love, O.V. Sinitsyna, et al. “Green” nanotechnologies: Synthesis of metal nanoparticles using plants. Acta Naturae, 2014, 6(1): 35–44.
[41]

R. Sahadevan, P. Sivakumar, P. Karthika, et al. Biosynthesis of silver nanoparticles from active compounds Quacetin–3-OBd-galactopyranoside containing plant extract and its antifungal application. The Asian Journal of Phamaceutical and Clinical Research, 2013, 6: 76−79.

[42]

A. Yaqub, S.A. Ditta, K.M. Anjum, et al. Comparative analysis of toxicity induced by different synthetic silver nanoparticles in albino mice. BioNanoScience, 2019, 9(3): 553−563. https://doi.org/10.1007/s12668-019-00642-y

[43]

C. Sharmila, R. Ranjith Kumar, B. Chandar Shekar. Psidium guajava: A novel plant in the synthesis of silver nanoparticles for biomedical applications. Asian Journal of Pharmaceutical and Clinical Research, 2018, 11(1): 341−345. https://doi.org/10.22159/ajpcr.2018.v11i1.21999

[44]

M.M.H. Khalil, E.H. Ismail, K.Z. El-Baghdady, et al. Green synthesis of silver nanoparticles using olive leaf extract and its antibacterial activity. Arabian Journal of Chemistry, 2014, 7(6): 1131−1139. https://doi.org/10.1016/j.arabjc.2013.04.007

[45]

M. Ijaz, M. Zafar, T. Iqbal. Green synthesis of silver nanoparticles by using various extracts: A review. Inorganic and Nano-Metal Chemistry, 2021, 51(5): 744−755. https://doi.org/10.1080/24701556.2020.1808680

[46]
T. Shankar, P. Karthiga, K. Swarnalatha, et al. Green synthesis of silver nanoparticles using Capsicum frutescence and its intensified activity against E. coli. Resource-Efficient Technologies, 2017, 3(3): 303–308.
[47]
F. Tulli, A.B. Cisneros, M.N. Gallucci, et al. Synthesis, properties, and uses of silver nanoparticles obtained from leaf extracts. Green Synthesis of Silver Nanomaterials: Elsevier, 2022: 317–357.
[48]

N. Ahmad, S. Sharma, M.K. Alam, et al. Rapid synthesis of silver nanoparticles using dried medicinal plant of basil. Colloids and Surfaces B:Biointerfaces, 2010, 81(1): 81−86. https://doi.org/10.1016/j.colsurfb.2010.06.029

[49]

V.A. Amaral, T.R. Alves, J.F. de Souza, et al. Phenolic compounds from Psidium guajava (Linn.) leaves: effect of the extraction-assisted method upon total phenolics content and antioxidant activity. Biointerface Research in Applied Chemistry, 2021, 11(2): 9346−9357. https://doi.org/10.33263/BRIAC112.93469357

[50]

P. Somchaidee, K. Tedsree. Green synthesis of high dispersion and narrow size distribution of zero-valent iron nanoparticles using guava leaf (Psidium guajava L) extract. Advances in Natural Sciences:Nanoscience and Nanotechnology, 2018, 9(3): 035006. https://doi.org/10.1088/2043-6254/aad5d7

[51]

C. Osorio, J.G. Carriazo, H. Barbosa. Thermal and structural study of guava (Psidium guajava L) powders obtained by two dehydration methods. Química Nova, 2011, 34(4): 636−640. https://doi.org/10.1590/S0100-40422011000400016

[52]
A. Taha, M. Shamsuddin. Biosynthesis of gold nanoparticles using psidium guajava leaf extract. Malaysian Journal of Fundamental and Applied Sciences, 2014, 9(3).
[53]

N. Jayaprakash, J.J. Vijaya, K. Kaviyarasu, et al. Green synthesis of Ag nanoparticles using Tamarind fruit extract for the antibacterial studies. Journal of Photochemistry and Photobiology B:Biology, 2017, 169: 178−185. https://doi.org/10.1016/j.jphotobiol.2017.03.013

[54]

B. Khodadadi, M. Bordbar, M. Nasrollahzadeh. Green synthesis of Pd nanoparticles at Apricot kernel shell substrate using Salvia hydrangea extract: catalytic activity for reduction of organic dyes. Journal of Colloid and Interface Science, 2017, 490: 1−10. https://doi.org/10.1016/j.jcis.2016.11.032

[55]

C.V. Restrepo, C.C. Villa. Synthesis of silver nanoparticles, influence of capping agents, and dependence on size and shape: A review. Environmental Nanotechnology,Monitoring &Management, 2021, 15: 100428. https://doi.org/10.1016/j.enmm.2021.100428

[56]

J. Fabrega, S.R. Fawcett, J.C. Renshaw, et al. Silver nanoparticle impact on bacterial growth: effect of pH, concentration, and organic matter. Environmental Science &Technology, 2009, 43(19): 7285−7290. https://doi.org/10.1021/es803259g

[57]

S.L. Palencia, A. Buelvas, M. Palencia. Interaction mechanisms of inorganic nanoparticles and biomolecular systems of microorganisms. Current Chemical Biology, 2015, 9(1): 10−22. https://doi.org/10.2174/2212796809666151022201811

[58]

S.M. Dizaj, F. Lotfipour, M. Barzegar-Jalali, et al. Antimicrobial activity of the metals and metal oxide nanoparticles. Materials Science and Engineering:C, 2014, 44: 278−284. https://doi.org/10.1016/j.msec.2014.08.031

[59]

Z. Ďuračková. Some Current insights into oxidative stress. Physiological Research, 2010, 59(4): 459−469. https://doi.org/10.33549/physiolres.931844

[60]

M.J. Piao, K.A. Kang, I.K. Lee, et al. Silver nanoparticles induce oxidative cell damage in human liver cells through inhibition of reduced glutathione and induction of mitochondria-involved apoptosis. Toxicology Letters, 2011, 201(1): 92−100. https://doi.org/10.1016/j.toxlet.2010.12.010

[61]
B. Halliwell, J.M.C. Gutteridge, Free Radicals in Biology and Medicine. 5th ed. Oxford University Press: USA, 2015.
[62]

S. Khorrami, A. Zarrabi, M. Khaleghi, et al. Selective cytotoxicity of green synthesized silver nanoparticles against the MCF-7 tumor cell line and their enhanced antioxidant and antimicrobial properties. International Journal of Nanomedicine, 2018, 13: 8013−8024. https://doi.org/10.2147/IJN.S189295

[63]

K. Gudikandula, S. Charya Maringanti. Synthesis of silver nanoparticles by chemical and biological methods and their antimicrobial properties. Journal of Experimental Nanoscience, 2016, 11(9): 714−721. https://doi.org/10.1080/17458080.2016.1139196

[64]

R.S. Priya, D. Geetha, P. Ramesh. Antioxidant activity of chemically synthesized AgNPs and biosynthesized Pongamia pinnata leaf extract mediated AgNPs–A comparative study. Ecotoxicology and Environmental Safety, 2016, 134: 308−318. https://doi.org/10.1016/j.ecoenv.2015.07.037

[65]

I. Ashok, R. Sheeladevi, D. Wankhar. Acute effect of aspartame-induced oxidative stress in Wistar albino rat brain. Journal of Biomedical Research, 2015, 29(5): 390−396. https://doi.org/10.7555%2FJBR.28.20120118

[66]

Y.-H. Lee, F.-Y. Cheng, H.-W. Chiu, et al. Cytotoxicity, oxidative stress, apoptosis and the autophagic effects of silver nanoparticles in mouse embryonic fibroblasts. Biomaterials, 2014, 35(16): 4706−4715. https://doi.org/10.1016/j.biomaterials.2014.02.021

[67]

P. Chairuangkitti, S. Lawanprasert, S. Roytrakul, et al. Silver nanoparticles induce toxicity in A549 cells via ROS-dependent and ROS-independent pathways. Toxicology in Vitro, 2013, 27(1): 330−338. https://doi.org/10.1016/j.tiv.2012.08.021

[68]

Y. Wu, Q.F. Zhou. Silver nanoparticles cause oxidative damage and histological changes in medaka (Oryzias latipes) after 14 days of exposure. Environmental Toxicology and Chemistry, 2013, 32(1): 165−173. https://doi.org/10.1002/etc.2038

[69]

E. Beytut, M. Aksakal. The effect of long-term supplemental dietary cadmium on lipid peroxidation and the antioxidant system in the liver and kidneys of rabbits. Turkish Journal of Veterinary &Animal Sciences, 2002, 26(5): 1055−1060.

[70]
K. Boulahia, P. Carol, S. Planchais, et al. Phaseolus vulgaris L. seedlings exposed to prometryn herbicide contaminated soil trigger an oxidative stress response. Journal of Agricultural and Food Chemistry, 2016, 64(16): 3150–3160.
[71]

P.J. Babu, A. Tirkey, T.J.M. Rao, et al. Conventional and nanotechnology based sensors for creatinine (a kidney biomarker) detection: A consolidated review. Analytical Biochemistry, 2022, 645: 114622. https://doi.org/10.1016/j.ab.2022.114622

[72]
S.H. Kadhim, M.M. Ubaid, H.K.M. Alboaklah. New biomarkers for diagnosing and treatment of kidney failure disease. kerbala journal of pharmaceutical sciences, 2022, 1(20).
[73]

S.H. Park, J.O. Lim, W.I. Kim, et al. Subchronic toxicity evaluation of aluminum oxide nanoparticles in rats following 28-day repeated oral administration. Biological Trace Element Research, 2022, 200(7): 3215−3226. https://doi.org/10.1007/s12011-021-02926-5

[74]

C. Recordati, M. De Maglie, S. Bianchessi, et al. Tissue distribution and acute toxicity of silver after single intravenous administration in mice: Nano-specific and size-dependent effects. Particle and Fibre Toxicology, 2016, 13: 12. https://doi.org/10.1186/s12989-016-0124-x

[75]
L. Stru&#380;yńska. Silver, Ag. In: Mammals and Birds as Bioindicators of Trace Element Contaminations in Terrestrial Environments. Cham: Springer, 2019: 655-691.
[76]

H.M. Jhanzab, A. Razzaq, Y. Bibi, et al. Proteomic analysis of the effect of inorganic and organic chemicals on silver nanoparticles in wheat. International Journal of Molecular Sciences, 2019, 20(4): 825. https://doi.org/10.3390/ijms20040825

[77]

T. Silva, L.R. Pokhrel, B. Dubey, et al. Particle size, surface charge and concentration dependent ecotoxicity of three organo-coated silver nanoparticles: comparison between general linear model-predicted and observed toxicity. Science of the Total Environment, 2014, 468: 968−976. https://doi.org/10.1016/j.scitotenv.2013.09.006

Nano Biomedicine and Engineering
Pages 225-238
Cite this article:
Yaqub A, Rashid M, Ditta SA, et al. In vivo Antioxidant Potential of Biogenic Silver Nanoparticles Synthesized from Psidium guajava L.. Nano Biomedicine and Engineering, 2023, 15(3): 225-238. https://doi.org/10.26599/NBE.2023.9290026

1288

Views

158

Downloads

3

Crossref

3

Scopus

Altmetrics

Received: 08 April 2023
Revised: 04 June 2023
Accepted: 16 June 2023
Published: 10 August 2023
© The Author(s) 2023.

This is an open-access article distributed under  the  terms  of  the  Creative  Commons  Attribution  4.0 International  License (CC BY) (http://creativecommons.org/licenses/by/4.0/), which  permits  unrestricted  use,  distribution,  and reproduction in any medium, provided the original author and source are credited.

Return