Application of <i>Aloe vera</i> Gel Blended Polymer-Collagen Scaffolds for Bone Tissue Engineering

Marwa Abdul Muhsien Hassan; Asmaa Hadi Mohammed; Ekhlas Majeed Hameed

doi:10.26599/NBE.2023.9290016

Nano Biomedicine and Engineering 2023, 15(2): 118-125 https://doi.org/10.26599/NBE.2023.9290016

Research Article |

Open Access | Issue | Published: 16 June 2023

Application of Aloe vera Gel Blended Polymer-Collagen Scaffolds for Bone Tissue Engineering

Show Author's Information Hide Author's Information Marwa Abdul Muhsien Hassan^¹(

), Asmaa Hadi Mohammed^², Ekhlas Majeed Hameed^³

1Department of Physics, College of Science, Mustansiriyah University, Baghdad, Iraq

2Department of Physics, College of Science, Al-Nahrain University, Baghdad, Iraq

3College of Medical, University of Wasit, Wasit, Iraq

Keywords:

bone tissue engineering, Aloe vera, polymer-collagen scaffolds

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Hassan MAM, Mohammed AH, Hameed EM. Application of Aloe vera Gel Blended Polymer-Collagen Scaffolds for Bone Tissue Engineering. Nano Biomedicine and Engineering, 2023, 15(2): 118-125. https://doi.org/10.26599/NBE.2023.9290016

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Abstract

Osteoblasts have an essential role in the process of bone formation, and polymer-collagen-Aloe vera (AV) is known to stimulate osteoblast proliferation and maturation. In this translational study, the effects of scaffolds on bone healing and the potential mechanisms responsible were investigated using an animal model of bone defects. Here, following surgical introduction of a bone defect in the proximal femurs of male rabbits, the left femur was implanted with scaffolds for 21 days, then compared to the right femur, which served as a control. According to histological analyses, the use of scaffolds did not result in hepatotoxicity or nephrotoxicity. In contrast to the control group, imaging using X-ray transmission and microcomputed tomography revealed that scaffold implantation boosted the bone repair. In addition, microcomputed tomographic and bone histomorphometric assays in the scaffold-treated group exposed an expansion in the formation of new trabecular bone. Furthermore, scaffold implantation resulted in a considerable increase in trabecular bone thickness but a decrease in the trabecular parameter factor. Following scaffold implantation, the quantities of alkaline phosphatase and osteocalcin, biomarkers capable of simulating bone development, were found to have gradually increased. Overall, this translational study found that scaffolds can improve bone repair by increasing trabecular bone creation via upregulation of Runx2-mediated alkaline phosphatase and osteocalcin gene expression. Our findings therefore suggest that scaffolds can be used to treat bone problems such as deformities and fractures.

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About this article

Application of Aloe vera Gel Blended Polymer-Collagen Scaffolds for Bone Tissue Engineering

Show Author's information Hide Author's Information Marwa Abdul Muhsien Hassan^¹(

), Asmaa Hadi Mohammed^², Ekhlas Majeed Hameed^³

1Department of Physics, College of Science, Mustansiriyah University, Baghdad, Iraq

2Department of Physics, College of Science, Al-Nahrain University, Baghdad, Iraq

3College of Medical, University of Wasit, Wasit, Iraq

Abstract

Keywords: bone tissue engineering, Aloe vera, polymer-collagen scaffolds

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Acknowledgements

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Publication history

Received: 22 May 2022

Revised: 18 February 2023

Accepted: 03 April 2023

Published: 16 June 2023

Issue date: June 2023

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Acknowledgements

CRediT author statement

Marwa Abdul Muhsien Hassan: Formal analysis, Funding acquisition, Writing (review and editing). Asmaa Hadi Mohammed: Supervision, Writing (original draft). Ekhlas Majeed Hameed: Methodology, Software.

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This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (CC BY) (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.