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Review Article | Open Access

Genome-wide studies of time of day in the brain: Design and analysis

Gang Wu1, Marc D. Ruben1, Yinyeng Lee1, Jiajia Li2, Michael E. Hughes2, John B. Hogenesch1( )
 Divisions of Human Genetics and Immunobiology, Center for Chronobiology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, 240 Albert Sabin Way, Cincinnati, OH 45229, U.S.A.
 Division of Pulmonary and Critical Care Medicine, Washington University School of Medicine, St. Louis, MO 63310, U.S.A.
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Abstract

Transcriptome profiling at different times of day is powerful for studying circadian regulation in model organisms and humans. To date, 24 h profiles from many tissue types suggest that about half of all genes are circadian-expressed somewhere in the body. However, few of these studies focused on the brain. Thus, despite known links between circadian disruption and neurological disease, we have virtually no mechanistic understanding. In the coming decade, we expect more genome-wide studies of time of day in different brain diseases, regions, and cell types. We expect just as many different approaches to the design and analysis of these studies. This review considers key principles of circadian tran scriptomics, with the goal of maximizing utility and reproducibility of future studies in the nervous system.

Keywords

circadiantranscriptomerhythmic analysisbrain

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Brain Science Advances
Volume 6 Issue 2,
June 2020
Pages 92-105
DOI: 10.26599/BSA.2020.9050005
Cite this article:
Wu G, Ruben MD, Lee Y, et al. Genome-wide studies of time of day in the brain: Design and analysis. Brain Science Advances, 2020, 6(2): 92-105. https://doi.org/10.26599/BSA.2020.9050005

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Received: 13 January 2020
Accepted: 27 February 2020
Published: 31 August 2020
© The authors 2020.

This article is published with open access at journals.sagepub.com/home/BSA

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